Patterns of maternal messenger RNA accumulation and adenylation during oogenesis in Urechis caupo.
Patterns of maternal messenger RNA accumulation and adenylation during oogenesis in Urechis caupo.
复制标题
Urechis caupo 卵子发生过程中母体信使 RNA 积累和腺苷酸化的模式。
DOI：
10.1016/0012-1606(86)90347-7
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Wilt,FH
中科院分区：
文献类型：
--
作者：
Rosenthal,ET;Wilt,FH
我们研究了尾刺尾虫卵母细胞卵子发生过程中母体mRNA的积累和腺苷酸化。利用体外翻译和cDNA探针分析特定的mRNA，表明不同的母体mRNA在卵子发生过程中以不同的模式积累。一类母体mRNA在整个卵子发生过程中积累，并在完全发育的卵母细胞中保持稳定水平。这些mRNA没有足够长的poly（A）尾来介导与卵母细胞中的oligo（dT）-纤维素的结合，但在受精后立即被迅速腺苷酸化。其他母体mRNA在生长中的卵母细胞中积累为poly（A）+RNA，并在完全生长的卵母细胞和胚胎中进行一些去腺苷化。这些mRNA中的一些在卵子发生的早期达到最高浓度，而另一些则在后期继续积累。许多在生长的卵母细胞中积累为poly（A）+RNA的mRNA在完全生长的卵母细胞中的浓度显著降低。
We have investigated the accumulation and adenylation of the maternal mRNA during oogenesis in the oocytes of the marine wormUrechis caupo. The analysis, usingin vitrotranslation and cDNA probes to assay for specific mRNAs, demonstrates that different maternal mRNAs accumulate with different patterns during oogenesis. One class of maternal mRNAs accumulates throughout oogenesis and remains at a steady level in the full-grown oocyte. These mRNAs do not have a poly(A) tail long enough to mediate binding to oligo(dT)-cellulose in oocytes, but are rapidly adenylated immediately following fertilization. The other maternal mRNAs accumulate in growing oocytes as poly(A)+RNA and undergo some deadenylation in full-grown oocytes and embryos. Some of these mRNAs attain their highest concentration fairly early in oogenesis, while others continue to accumulate during later stages. Many of the mRNAs that accumulate as poly(A)+RNA in growing oocytes diminish dramatically in concentration in full-grown oocytes.