Cloning, characterization, and genetic mapping of the rat type 2 angiotensin II receptor gene.
Cloning, characterization, and genetic mapping of the rat type 2 angiotensin II receptor gene.
复制标题
大鼠 2 型血管紧张素 II 受体基因的克隆、表征和遗传图谱。
DOI：
10.1161/01.hyp.26.6.998
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Jacob,HJ
中科院分区：
文献类型：
--
作者：
Koike,G;Winer,ES;Horiuchi,M;Brown,DM;Szpirer,C;Dzau,VJ;Jacob,HJ
肾素-血管紧张素系统在血压稳态中发挥着重要作用，但2型血管紧张素II受体（AT2R）的贡献仍不清楚。 AT2R基因已被定位到人和大鼠的X染色体上的报道，以及先前关于X染色体上负责血压升高的基因Bp3的报道表明，大鼠AT2R基因(Agtr2)可能就是该基因。为了阐明Agtr2是否是Bp3，克隆了Agtr2。鉴定了该基因 3' 侧翼区域中的简单序列重复，并将其用作遗传标记，将 Agtr2 映射到 X 染色体上雄激素受体基因座远端 18.1 cM 处。该图位置位于 Bp3 报告的置信区间之外，表明 Agtr2 不可能是 Bp3。然而，这些数据将加强对 AT2R 生物学以及 X 染色体的研究。
The renin-angiotensin system plays an important role in blood pressure homeostasis, but the contribution of the type 2 angiotensin II receptor (AT2R) is still unclear. The reports that the AT2R gene has been mapped to the X chromosome in human and rat and the previous report of a gene,Bp3,on the X chromosome responsible for an increase in blood pressure have suggested that the rat AT2R gene(Agtr2)could be this gene. To elucidate whetherAgtr2isBp3, Agtr2was cloned. A simple sequence repeat in the 3′-flanking region of this gene was identified and used as a genetic marker to mapAgtr2to the X chromosome at 18.1 cM distal to the androgen receptor locus. This map position is outside the confidence interval reported forBp3,demonstrating thatAgtr2cannot beBp3. However, these data will enhance the research into the AT2R biology as well as the study of the X chromosome.