Comparison of line-scanned and point-scanned dual-axis confocal microscope performance.
Comparison of line-scanned and point-scanned dual-axis confocal microscope performance.
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线扫描和点扫描双轴共焦显微镜性能比较。
DOI：
10.1364/ol.38.005280
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发表时间：
2013-12-15
期刊：
影响因子：
3.6
通讯作者：
Liu JT
中科院分区：
文献类型：
--
作者：
Wang D;Chen Y;Wang Y;Liu JT
与传统的单轴显微镜相比，点扫描双轴共焦 (PS-DAC) 显微镜已被证明具有卓越的抑制离焦光和多重散射光的能力。然而，逐点数据收集导致的低帧速率（通常< 5 Hz）使得这些系统容易受到运动伪影的影响。虽然视频速率点扫描共焦显微镜是可能的，但线扫描双轴共焦 (LS-DAC) 显微镜提供了一种通过逐行数据收集实现高速成像的更简单方法，但由于沿一维共焦的损失而牺牲了对比度。在这里，我们评估了具有相同空间分辨率的 LS-DAC 和 PS-DAC 显微镜之间的性能权衡。 LS-DAC 和 PS-DAC 显微镜与组织模型在反射模式下的表征实验与系统蒙特卡罗散射模拟的结果相匹配。使用分辨率匹配的 LS-DAC 和 PS-DAC 显微镜获得的小鼠脑血管系统的荧光图像证明了 LS-DAC 和 PS-DAC 显微镜在浅深度的性能相当。
The point-scanned dual-axis confocal (PS-DAC) microscope has been shown to exhibit a superior capability to reject out-of-focus and multiply scattered light in comparison to its conventional single-axis counterpart. However, the slow frame rate (typically < 5 Hz) resulting from point-by-point data collection makes these systems vulnerable to motion artifacts. While video-rate point-scanned confocal microscopy is possible, a line-scanned dual-axis confocal (LS-DAC) microscope provides a simpler means of achieving high-speed imaging through line-by-line data collection, but sacrifices contrast due to a loss of confocality along one dimension. Here we evaluate the performance tradeoffs between a LS-DAC and PS-DAC microscope with identical spatial resolutions. Characterization experiments of the LS-DAC and PS-DAC microscopes with tissue phantoms, in reflectance mode, are shown to match results from Monte-Carlo scattering simulations of the systems. Fluorescence images of mouse brain vasculature, obtained using resolution-matched LS-DAC and PS-DAC microscopes, demonstrate the comparable performance of LS-DAC and PS-DAC microscopy at shallow depths.