Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)
Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)
复制标题
野生番茄 (Solanum Pennellii) 中 ZPR1 基因的鉴定和表达模式
DOI：
10.1007/s11105-012-0509-4
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发表时间：
2013-04-01
影响因子：
2.1
通讯作者：
Li, Hanxia
中科院分区：
文献类型：
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作者：
Li, Jinhua;Wei Sima;Li, Hanxia
已知哺乳动物锌指蛋白 ZPR1 在细胞周期进程中至关重要，但其在植物中的同源物仍有待鉴定。在这里，我们从野生番茄品种Solanum pennelliiLA0716中分离出SpZPR1，并发现其蛋白质序列与其他生物体的ZPR1基因具有同源性。SpZPR1mRNA被发现响应ABA（脱落酸）处理和热应激而在番茄顶端分生组织中积累。酵母中的反式激活活性测定表明，SpZPR1 作为转录激活剂发挥作用，而我们的凝胶电泳迁移率变动测定的结果表明，SpZPR1 能够在体外结合 ABRE（ABA 响应元件）序列。我们的研究首次提供证据表明SpZPR1参与ABA信号网络并在植物细胞发育和非生物胁迫反应中发挥潜在作用。
The mammalian zinc finger protein ZPR1 is known to be essential in cell cycle progression, but its homolog in plants remains to be identified. Here, we isolatedSpZPR1from the wild tomato speciesSolanum pennelliiLA0716 and found that its protein sequence shares homology with ZPR1 genes from other organisms.SpZPR1mRNA was found to accumulate in tomato apical meristem in response to ABA (abscisic acid) treatment and heat stress. A transactivation activity assay in yeast revealed that SpZPR1 functions as a transcriptional activator, while the results of our gel electrophoresis mobility shift assay showed that SpZPR1 was able to bind the ABRE (ABA-responsive element) sequence in vitro. Our study is the first to provide evidence thatSpZPR1is involved in the ABA signaling network and plays a potential role in plant cell development and abiotic stress response.