Full‐length Numt analysis provides evidence for hybridization between the Asian colobine genera Trachypithecus and Semnopithecus
Full‐length Numt analysis provides evidence for hybridization between the Asian colobine genera Trachypithecus and Semnopithecus
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DOI：
10.1002/ajp.22419
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发表时间：
2015-08
影响因子：
2.4
通讯作者：
Boshi Wang;Xuming Zhou;Fanglei Shi;Zhijin Liu;C. Roos;P. Garber;Ming Li;Huijuan Pan
中科院分区：
文献类型：
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作者：
Boshi Wang;Xuming Zhou;Fanglei Shi;Zhijin Liu;C. Roos;P. Garber;Ming Li;Huijuan Pan
用不同的分子标记进行推断时，由于拓扑学上的不一致，导致该属的系统发育地位尚未确定。虽然一些研究提出了文氏猿属和管猿属之间的杂交来解释这种不一致，但还没有确凿的证据表明杂交是存在的。为了解决这个问题，我们使用DNA步行和长程聚合酶链式反应(Long-Range-Range PCR)描述了存在于锥体动物中的核线粒体DNA(Numt)片段，其长度超过15kb，约占整个线粒体基因组的92%。我们评估了该Numt在其他16种Colobine物种中的存在，包括Trachypithecus属4种，Semnopithecus属6种，以及Colobinae亚科其他6属的代表性物种。我们没有在其他任何一种孔雀中检测到Numt序列，除了与毛细管藻密切相关的短毛网眼线虫。该Numt与Semnopithecus属的姊妹关系表明，该Numt是由Semnopithecus属的mt基因组衍生而来，并通过单向导入杂交的方式侵入毛刺属的核基因组。这些结果为长尾猿和长尾猿之间杂交的存在提供了最确凿的证据。上午好。J.Primatol。2015年，77：901-910。©2015 Wiley期刊，Inc.
The phylogenetic position of the genus Semnopithecusis unresolved because of topological incongruence when inferred using different molecular markers. Although some studies proposed hybridization between the genera Semnopithecus and Trachypithecus to explain the discordance, no conclusive evidence for hybridization has been identified. To address this issue, we used DNA walking and long‐range PCR to describe a nuclear mitochondrial DNA (Numt) segment present in Trachypithecus pileatus which extends over more than 15 kb, and represents approximately 92% of the entire mitochondrial genome. We assessed the presence of this Numt in 16 other colobine species, including four species of the genus Trachypithecus, six species of the genus Semnopithecus, and representative species of six other genera belonging to the subfamily Colobinae. We failed to detect a Numt sequence in any of the other colobine species except for T. shortridgei, which is closely related to T. pileatus. The sister relationship of this Numt within the genus Semnopithecus suggests that it was derived from the mt genome of the genus Semnopithecus and invaded the nuclear genome of T. pileatus by unidirectional introgression hybridization. These results offer the most conclusive evidence for the existence of hybridization between Semnopithecus and Trachypithecus. Am. J. Primatol. 77:901–910, 2015. © 2015 Wiley Periodicals, Inc.