Gene expression in Xenopus embryogenesis.
Gene expression in Xenopus embryogenesis.
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发表时间：
1985-11
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通讯作者：
I. Dawid;S. Haynes;M. Jamrich;E. Jónás;S. Miyatani;T. Sargent;J. Winkles
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作者：
I. Dawid;S. Haynes;M. Jamrich;E. Jónás;S. Miyatani;T. Sargent;J. Winkles
本文讨论了非洲爪哇卵母细胞中大分子的储存以及早期胚胎发育过程中先前未表达的基因的激活。卵子中积累的大量复杂的Poly(A)+RNA为卵裂胚胎提供了足够维持几个小时蛋白质合成的mRNA。在中期胚泡转变(MBT)时，基因活动开始导致各种RNA类分子的合成和积累，包括tRNAs、rRNAs、mRNAs和线粒体RNAs。在原肠形成时，Poly(A)+RNA群体在性质上仍与鸡蛋相似，但一些鸡蛋RNA中不存在的序列已经积累。通过使用消减cDNA克隆程序，我们已经准备了一个代表MBT和原肠胚之间第一次激活的基因的序列文库。对其中几个克隆的研究表明，这类基因的活性仅限于胚胎和蝌蚪阶段。
This article considers some aspects of the storage of macromolecules in the oocyte of Xenopus laevis and the activation of previously unexpressed genes during early embryogenesis. The large quantity and complex nature of poly(A)+ RNA accumulated in the egg provides the cleavage embryo with a supply of mRNA sufficient to sustain protein synthesis for several hours of development. Onset of gene activity at the midblastula transition (MBT) leads to the synthesis and accumulation of molecules of various RNA classes, including tRNAs, rRNAs, mRNAs and mitochondrial RNAs. At gastrulation the poly(A)+ RNA population is still qualitatively similar to that of the egg but some sequences not present in egg RNA have accumulated by this time. Through the use of a subtractive cDNA cloning procedure we have prepared a library of sequences that represent genes activated for the first time between MBT and gastrula. A study of several of these cDNA clones suggests that genes in this class are restricted in their activity to embryonic and tadpole stages.