Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract
Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract
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DOI：
10.1021/jf801744r
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发表时间：
2008-09-10
影响因子：
6.1
通讯作者：
Goya, Luis
中科院分区：
文献类型：
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作者：
Martin, Maria Angeles;Ramos, Sonia;Goya, Luis
可可含有丰富的黄烷醇和原花青素低聚物，具有抗氧化性能，可防止氧化和硝化。本研究探讨了可可多酚提取物对叔丁基氢过氧化氢(t-BOOH)诱导的体外培养的人肝癌细胞株HepG2细胞活力和抗氧化防御的潜在保护作用。用0.05-50微克/毫升的可可多酚提取物(CPE)处理细胞2或20小时，可完全阻止t-BOOH处理引起的细胞损伤和抗氧化酶活性的增强。此外，CPE可部分恢复t-BOOH引起的HepG2细胞内GSH水平的降低。CPE可剂量依赖性地抑制t-BOOH诱导的细胞内活性氧自由基(ROS)的增加。这些结果表明，CPE(在生理浓度范围内)对培养的HepG2细胞具有明显的抗氧化保护作用。
Cocoa is a rich source of flavanols and procyanidin oligomers with antioxiclative properties, providing protection against oxidation and nitration. The present study investigated the potential protective effect of a polyphenolic extract from cocoa on cell viability and antioxidant defenses of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Pretreatment of cells with 0.05-50 mu g/mL of cocoa polyphenolic extract (CPE) for 2 or 20 h completely prevented cell damage and enhanced activity of antioxidant enzymes induced by a treatment with t-BOOH. Moreover, lower levels of GSH caused by t-BOOH in HepG2 cells were partly recovered by a pretreatment with CPE. Increased reactive oxygen species (ROS) induced by t-BOOH was dose-dependently prevented when cells were pretreated for 2 or 20 h with CPE. These results show that treatment of HepG2 in culture with CPE (within the physiological range of concentrations) confers a significant protection against oxidation to the cells.