Morphological identities of two different marine stramenopile environmental sequence clades: Bicosoeca kenaiensis (Hilliard 1971) and Cantina marsupialis (Larsen and Patterson, 1990) gen. nov., comb. nov.
Morphological identities of two different marine stramenopile environmental sequence clades: Bicosoeca kenaiensis (Hilliard 1971) and Cantina marsupialis (Larsen and Patterson, 1990) gen. nov., comb. nov.
复制标题
两个不同海洋原生藻菌环境序列分支的形态特征：Bicosoeca kenaiensis (Hilliard 1971) 和 Cantina marsupialis (Larsen 和 Patterson, 1990) gen.
DOI：
10.1111/jeu.12207
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发表时间：
2015
影响因子：
2.2
通讯作者：
Brian S. Leander
中科院分区：
文献类型：
--
作者：
Naoji Yubuki;Tomas Panek;Akinori Yabuki;Ivan Cepicka;Kiyotaka Takishita;Yuji Inagaki;Brian S. Leander
尽管环境DNA调查提高了我们对生物多样性的理解，但由于缺乏相关的形态特征和活的文化，对未知谱系的解释受到限制。无法确定的海洋草纲动物的谱系被称为“桅杆支系”。已经识别了25个桅杆分支：MAST-1到MAST-25；其中7个随后被丢弃，因为表示这些分支的序列被发现或者(1)是嵌合的，或者(2)在先前描述的分类群中附属。18个桅杆分支仍然没有蜂窝身份。此外，废弃的“MAST-13”在不同的研究中被用于指代两个不同的环境层序分支。在建立了代表两个不同种类的异养稻草藻的四种培养物，然后对它们的形态和分子系统发育位置进行了表征后，我们确定这两个不同的物种代表了两个不同的Mast-13分支：(1)带刺洛里卡的Bicosoeca kenaisis和(2)以前被命名为“袋袋自助餐厅”的微嗜气鞭毛虫。这两个物种以前都是用光学显微镜描述的；在本研究之前，这些物种没有培养、超微结构数据或DNA序列。三个不同的“袋毛虫”分离物的分子系统发育位置与Cafeteria的模式种关系不大，因此，我们为这些分离物建立了一个新属--坎提纳根。11月
Although environmental DNA surveys improve our understanding of biodiversity, interpretation of unidentified lineages is limited by the absence of associated morphological traits and living cultures. Unidentified lineages of marine stramenopiles are called “MAST clades”. Twenty‐five MAST clades have been recognized: MAST‐1 through MAST‐25; seven of these have been subsequently discarded because the sequences representing those clades were found to either (1) be chimeric or (2) affiliate within previously described taxonomic groups. Eighteen MAST clades remain without a cellular identity. Moreover, the discarded “MAST‐13” has been used in different studies to refer to two different environmental sequence clades. After establishing four cultures representing two different species of heterotrophic stramenopiles and then characterizing their morphology and molecular phylogenetic positions, we determined that the two different species represented the two different MAST‐13 clades: (1) a lorica‐bearingBicosoeca kenaiensisand (2) a microaerophilic flagellate previously named “Cafeteria marsupialis”. Both species were previously described with only light microscopy; no cultures, ultrastructural data or DNA sequences were available from these species prior to this study. The molecular phylogenetic position of three different “C. marsupialis” isolates was not closely related to the type species ofCafeteria; therefore, we established a new genus for these isolates,Cantinagen. nov.