EFFECTS OF SIDEROPHORES ON THE GROWTH OF PSEUDOMONAS-AERUGINOSA IN HUMAN-SERUM AND TRANSFERRIN
EFFECTS OF SIDEROPHORES ON THE GROWTH OF PSEUDOMONAS-AERUGINOSA IN HUMAN-SERUM AND TRANSFERRIN
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DOI：
10.1128/iai.49.1.132-140.1985
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
COX, CD
中科院分区：
文献类型：
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作者：
ANKENBAUER, R;SRIYOSACHATI, S;COX, CD
铜绿假单胞菌、绿脓菌素和脓毒蛋白产生的铁载体的组合可显着刺激该细菌在含有人转铁蛋白的培养基中的生长。当单独添加每种铁载体时观察到的生长刺激量仅略低于使用组合时观察到的量。分离菌株 PAO1 的铁载体缺陷突变体，以测试铁载体的产生对转铁蛋白和人血清中生长的影响。富含脓毒素（Pvd+）、缺乏脓毒素（Pch-）的菌株（IA5）与亲本（PAO1）一样生长，后者产生两种铁粒细胞。另一方面，Pvd-Pch+菌株(211-5)的生长严重迟缓，类似于缺乏两种铁载体产生的突变体(IA1)所证明的情况，但与菌株IA1相比，在生长曲线的后期具有加速的对数期。然而，在人血清中孵育期间，Pvd-Pch+菌株(211-5)相对于Pvd-Pch-菌株IA1没有明显的优势。铜绿假单胞菌菌株无法在葡萄糖最低培养基中产生绿脓杆菌素，这可以解释211-5在该培养基和人血清中生长不良的原因。 211-5 菌株在允许绿脓杆菌素合成的培养基中比 IA1 菌株生长得好得多，但它仍然不如 Pvd+ Pch- 菌株 (IA5) 生长得好。因此，脓毒蛋白似乎是人血清中生长最重要的铁载体。
A combination of the siderophores produced by Pseudomonas aeruginosa, pyochelin and pyoverdin, dramatically stimulates the growth of this bacterium in medium containing human transferrin. The amount of growth stimulation observed when each siderophore was added alone was only slightly less than the amount observed with the combination. Siderophore-defective mutants of strain PAO1 were isolated to test the effects of siderophore production on growth in transferrin and human serum. The pyoverdin-proficient (Pvd+), pyochelin-deficient (Pch-) strain (IA5) grows just as well as the parent (PAO1), which produces both siderohores. On the other hand, the Pvd- Pch+ strain (211-5) has severely retarded growth, similar to that demonstrated by a mutant lacking production of both siderophores (IA1), but has an accelerated log phase compared with strain IA1 at the later stages of the growth curve. However, the Pvd- Pch+ strain (211-5) had no observable advantage over the Pvd- Pch- strain, IA1, during incubation in human serum. The inability of P. aeruginosa strains to produce pyochelin in glucose-minimal medium may explain the poor growth of 211-5 in this medium and in human serum. The 211-5 strain grows much better than the IA1 strain in the medium that allows pyochelin synthesis, but it still does not grow as well as the Pvd+ Pch- strain (IA5). Therefore, pyoverdin appears to be the most important siderophore for growth in human serum.