Occurrence of tetrodotoxin in a gastropod mollusk, "araregai" Niotha clathrata.
Occurrence of tetrodotoxin in a gastropod mollusk, "araregai" Niotha clathrata.
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河豚毒素在腹足类软体动物“araregai”Niotha clathrata 中的存在。
DOI：
10.2331/suisan.50.2099
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发表时间：
1984
影响因子：
0.4
通讯作者：
K. Hashimoto
中科院分区：
文献类型：
--
作者：
J. Jeon;H. Harita;M. Nara;T. Noguchi;J. Maruyama;K. Hashimoto
在小型腹足类软体动物“araregai”Niotha clathrata 中检测到麻痹毒性。通过一种方法从腹足动物的消化腺中部分纯化毒素，该方法主要包括活性炭处理，然后在 CM-Sephadex C-25 和 Bio-Rex 70 上进行色谱分析。如此纯化的阿拉雷盖毒素在薄层色谱和电泳行为中与河豚毒素无法区分。此外，对阿拉雷盖毒素碱解产物的气相色谱-质谱分析表明存在河豚毒素特有的喹唑啉骨架。根据这些结果，阿拉雷盖毒素被鉴定为河豚毒素。
Paralytic toxicity was detected in a small gastropod mollusk, “araregai” Niotha clathrata. The toxin was partially purified from digestive glands of the gastropod by a method which essentially consisted of activated charcoal treatment, followed by chromatography on CM-Sephadex C-25 and Bio-Rex 70. Araregai toxin thus purified was indistinguishable from tetrodotoxin in thin-layer chromatographic and electrophoretic behaviors. In addition, gas chromatography-mass spectrometry of the alkali hydrolyzate of araregai toxin indicated the presence of quinazoline skeleton specific to tetrodotoxin. Araregai toxin was identified as tetrodotoxin from these results.