Real-time voltammetric detection of cocaine-induced dopamine changes in the striatum of freely moving mice
Real-time voltammetric detection of cocaine-induced dopamine changes in the striatum of freely moving mice
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DOI：
10.1016/j.neulet.2009.10.025
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发表时间：
2009-12-25
影响因子：
2.5
通讯作者：
Budygin, Evgeny A.
中科院分区：
文献类型：
--
作者：
Oleson, Erik B.;Salek, Jonathan;Budygin, Evgeny A.
在目前的伏安研究中，我们实时表征了可卡因诱导的自由活动小鼠纹状体内诱发的多巴胺释放和摄取的变化。可卡因引起明显的多巴胺摄取抑制，表现为明显的K-m变化，在单次注射(15 mg/kg)后20min作用最强。摄取的变化与每个刺激脉冲诱发的多巴胺释放增加是平行的，这表明在注射可卡因后，这两个参数之间存在高度相关性。可卡因对常用的C57BL/6小鼠品系纹状体多巴胺传递的影响的初步表征为未来使用遗传小鼠模型进行伏安研究提供了基础。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
In the present voltammetric study, we have characterized cocaine-induced changes in evoked dopamine release and uptake in the striatum of freely moving mice in real time. Cocaine induced marked dopamine uptake inhibition measured as apparent K-m changes, producing a maximal effect 20 min following a single injection (15 mg/kg, i.p.). Changes in uptake were paralleled by increases in evoked dopamine release per stimulus pulse, revealing a high correlation between these two parameters following cocaine administration. This initial characterization of cocaine effects on striatal dopamine transmission in the commonly used C57BL/6 mouse strain provides a basis for future voltammetric studies using genetic mouse models. (C) 2009 Elsevier Ireland Ltd. All rights reserved.