Neovascularization and bone regeneration by implantation of autologous bone marrow mononuclear cells
Neovascularization and bone regeneration by implantation of autologous bone marrow mononuclear cells
复制标题
DOI：
10.1016/j.biomaterials.2004.11.032
复制
发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Ochi, M
中科院分区：
文献类型：
--
作者：
Hisatome, T;Yasunaga, Y;Ochi, M
本研究观察了自体骨髓单个核细胞（BM-MNCs）移植对兔股骨缺损的新生血管和骨髓骨再生的影响。制备了碱性成纤维细胞生长因子（bFGF）明胶微球，用于bFGF的控释。为了评价植入BM-MNCs的体内效应，我们在兔股骨内侧髁上制造骨缺损，并将5 × 10（6）荧光标记的自体BM-MNCs与含有10 μ g bFGF的明胶微球一起植入去端胶原凝胶支架中。将四个实验组，即Atelocollagengel（Cot）、Cot + 5 × 10（6）BM-MNCs、Cot + 10 μ gbFGF和Cot + 5 × 10（6）BM-MNCs + 10 μ gbFGF，以Atelocollagengel作为支架植入制备的缺损部位。自体BM-MNCs表达CD 31，一种内皮谱系细胞标志物，并在植入后2周在植入部位诱导有效的新生血管形成。与其他组相比，Col + BM-MNCs + bFGF中的毛细血管密度显著较大。这种组合也在8周后增强了骨缺损的再生，其程度显著大于BM-MNCs或bFGF本身。总之，这些发现表明BM-MNCs和bFGF明胶水凝胶的组合增强了新血管形成和骨诱导能力，导致骨再生。(c)2004 Elsevier Ltd.保留所有权利。
We examined whether transplantation of autologous bone marrow mononuclear cells (BM-MNCs) can augment neovascularization and bone regeneration of bone marrow in femoral bone defects of rabbits. Gelatin microspheres containing basic fibroblast growth factor (bFGF) were prepared for the controlled release of bFGF. To evaluate the in vivo effect of implanted BM-MNCs, we created bone defects in the rabbit medial femoral condyle, and implanted into them 5 x 10(6) fluorescent-labeled autologous BM-MNCs together with gelatin microspheres containing 10 mu g bFGF on an atelocollagen gel scaffold. The four experimental groups, which were Atelocollagen gel (Cot), Cot + 5 x 10(6) BM-MNCs, Cot + 10 mu g bFGF, and Cot + 5 x 10(6) BM-MNCs + 10 mu g bFGF, were implanted into the sites of the prepared defects using Atelocollagen gel as a scaffold. The autologous BM-MNCs expressed CD31, an endothelial lineage cell marker, and induced efficient neovascularization at the implanted site 2 weeks after implantation. Capillary density in Col + BM-MNCs + bFGF was significantly large compared with other groups. This combination also enhanced regeneration of the bone defect after 8 weeks to a significantly greater extent than either BM-MNCs or bFGF on their own. In summary, these findings demonstrate that a combination of BM-MNCs and bFGF gelatin hydrogel enhance the neovascularization and the osteoinductive ability, resulting in bone regeneration. (c) 2004 Elsevier Ltd. All rights reserved.