Fire blight resistance of Malus xarnoldiana is controlled by a quantitative trait locus located at the distal end of linkage group 12
Fire blight resistance of Malus xarnoldiana is controlled by a quantitative trait locus located at the distal end of linkage group 12
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DOI：
10.1007/s10658-017-1152-6
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发表时间：
2017-08-01
影响因子：
1.8
通讯作者：
Malnoy, Mickael
中科院分区：
文献类型：
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作者：
Emeriewen, Ofere Francis;Peil, Andreas;Malnoy, Mickael
梨火疫病菌是引起火疫病的病原体，火疫病是影响全世界仁果生产的最具破坏性的细菌性疾病。到目前为止，尽管已经鉴定了几个数量性状基因座（QTL），但在苹果属中仅报道了一种功能上经过验证的抗这种疾病的基因。由于一些高度攻击性的菌株能够克服苹果属中所描述的抗性，包括唯一分离的和功能上证明的抗性基因的抗性，因此识别更多可用于建立金字塔形和持久抗性的抗性供体非常重要。对来自 Malus xdomestica 品种 Idared 和野苹果品种 M. xarnoldiana 杂交的 F-1 后代进行了数量性状基因座分析。后代被人工接种了两种梨火梨菌株——中等攻击性菌株 Ea222_JKI 和高度攻击性加拿大菌株 Ea3049。在 M. xarnoldiana 连锁群 (LG) 12 的远端鉴定出 QTL，解释了接种 Ea222_JKI 的后代中超过 50% 的表型变异。使用接种 Ea3049 的后代的表型数据还鉴定了 LG12 上的显着 QTL 效应。 M. xarnoldiana (FB_Mar12) 的火疫病抗性 QTL 似乎位于苹果基因组 LG12 上 SSR 标记 Hi07f01 下方与 Evereste 和/或 M. floribunda 克隆 821 相似的位置。这里给出的结果表明该 QTL 可能与 LG12 上其他报道的火疫病 QTL 等位。然而，由于该野苹果对Ea3049表现出显着的抗性效果，因此有必要进行更多的研究，以进一步阐明该野苹果的抗性机制。
Erwinia amylovora is the pathogen which incites fire blight - the most devastating bacterial disease affecting pome fruit production worldwide. So far, only one functionally proven gene for resistance to this disease has been reported in Malus, even though several quantitative trait loci (QTLs) have been identified. Since some highly aggressive strains are capable of overcoming described resistances in Malus, including that of the only isolated and functionally-proven resistance gene, it is important to identify more resistance donors useful for the establishment of pyramided and durable resistance. Quantitative trait locus analyses were performed on F-1 progenies derived from a cross between the Malus xdomestica cultivar, Idared, and the wild apple species M. xarnoldiana. Progenies were artificially inoculated with two strains of E. amylovora - the moderately aggressive strain Ea222_JKI, and the highly aggressive Canadian strain, Ea3049. A QTL was identified at the distal end of linkage group (LG) 12 of M. xarnoldiana explaining over 50% of the phenotypic variation among the progenies inoculated with Ea222_JKI. A significant QTL effect was also identified on LG12 using the phenotypic data of the progenies inoculated with Ea3049. The fire blight resistance QTL of M. xarnoldiana (FB_Mar12) appeared to be located at a similar position as those of Evereste and/or M. floribunda clone 821 below the SSR marker Hi07f01 on LG12 of the apple genome. The results presented here suggest that this QTL could be allelic with the other reported fire blight QTLs on LG12. Nevertheless, it is imperative to perform more studies aimed at further elucidating the resistance mechanism of this wild apple species since it displays a significant resistance effect to Ea3049.