AUTOMATED MICROBIOLOGICAL METHOD FOR MEASUREMENT OF VITAMIN-B12
AUTOMATED MICROBIOLOGICAL METHOD FOR MEASUREMENT OF VITAMIN-B12
复制标题
DOI：
10.1136/jcp.26.7.494
复制
发表时间：
1973-01-01
影响因子：
3.4
通讯作者：
KELLY, A
中科院分区：
文献类型：
--
作者：
DAVIS, RE;MOULTON, J;KELLY, A
本文介绍了一种维生素B_（12）的自动微生物测定方法。使用抗氯霉素的莱氏乳杆菌菌株作为试验微生物，这消除了灭菌或无菌添加的需要。在加热制剂以释放维生素B12之前，通过用谷氨酸/苹果酸溶液稀释血清来避免血清蛋白沉淀。测试可以以每小时80次的速度设置，孵育后可以以每小时160次的速度读取。通过将编程的电子计算器直接与用于测定的Mecolab M连接，结果直接以ng/l打印。
An automated method for the microbiological assay of vitamin B12is described. A chloramphenicol-resistant strain ofLactobacillus leichmanniiis used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitamin B12. Tests can be set up at a rate of 80 an hour and after incubation they can be read at 160 an hour. By interfacing a programmed electronic calculator directly with the Mecolab M which is used for the assays, results are printed directly in ng/l.