Autoantibodies against glucuronosyltransferases differ between viral hepatitis and autoimmune hepatitis
Autoantibodies against glucuronosyltransferases differ between viral hepatitis and autoimmune hepatitis
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DOI：
10.1016/s0016-5085(96)70020-3
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发表时间：
1996-12-01
期刊：
影响因子：
29.4
通讯作者：
Manns, MP
中科院分区：
文献类型：
--
作者：
Strassburg, CP;ObermayerStraub, P;Manns, MP
Background & Aim: Approximately 13% of patients with chronic hepatitis D virus (HDV) infection have liver-kidney microsomal antibodies type 3 (LKM-3) directed against family 1 uridine 5'-diphosphate-glucuronosyl-transferases (UGT-1), The aim of this study was to characterize the prevalence and specificity of LKM-3 by recombinant antigen testing systems. Methods:Enzyme linked immunosorbent assay (ELISA) and Western blot were performed using baculovirus-generated human UGT-1.1 and -1,6 and rabbit UGT-1.6. Sera from patients with HDV (n = 50), autoimmune hepatitis (AIH) type 2 (n = 50), hepatitis B virus (n = 26), hepatitis C virus (HCV) (n = 25), and LKM-1 autoantibody-positive HCV (n = 14) and sera from normal controls (n = 50) and Italian patients with HDV and known LKM-3 autoantibodies were studied. Results: Six percent of patients with HDV from Germany and 8% of patients with type 2 AIH had LKM-3. Sera from Italian patients with HDV and patients with AIH type 2 recognized all three recombinant UGT-1. HDV sera from Germany selectively recognized human UGT-1. LKM-3 liters were lower in HDV than in AIH, One patient with AIH had LKM-3 as the only marker of AIH, Conclusions: This study indicates a molecular target and titer difference of LKM-3 autoantibodies in German subjects with HDV and AIH, It also suggests a geographic target and titer difference of LKM-3 in HDV. LKM-3 are identified as a rare and previously undescribed independent marker of AIH.
