TYPING OF HEPATITIS-C VIRUS ISOLATES AND CHARACTERIZATION OF NEW SUBTYPES USING A LINE PROBE ASSAY
TYPING OF HEPATITIS-C VIRUS ISOLATES AND CHARACTERIZATION OF NEW SUBTYPES USING A LINE PROBE ASSAY
复制标题
DOI：
10.1099/0022-1317-74-6-1093
复制
发表时间：
1993-06-01
影响因子：
3.8
通讯作者：
MAERTENS, G
中科院分区：
文献类型：
--
作者：
STUYVER, L;ROSSAU, R;MAERTENS, G
根据丙型肝炎病毒（HCV）不同基因型5'端非翻译区的变异，建立了一种快速、简便地检测HCV基因型及其亚型的反向杂交法--线性探针法（利帕）。使用这种方法，61 PCR阳性的巴西HCV血清分型。在血清中，33%有1型HCV感染，38%有1b型（与HCV-J相关），1.5%有2a型（与HC-J6相关），24.5%有3型（与E-b1和HCV-T相关），3%的血清是合并感染。使用来自比利时和荷兰的13份血清进一步评价了该测定格式，并且所有这些血清都可以被分类。两个日本血清池被归类为2a型或与1b型和2a型共感染，但未检测到2b型序列。另外8份PCR阳性血清来自布隆迪和加蓬。这些非洲病毒的5'非翻译区序列与先前描述的三种类型有很大差异。因此，这些分离株暂时归类为4型。在目前使用的第二代抗体确认试验中，这些血清和其他一些非1型血清的反应性通常弱于1型分离株。
A reverse-hybridization assay, the line probe assay (LiPA), based on variations found in the 5' untranslated regions of the different hepatitis C virus (HCV) genotypes was developed, permitting simple and fast determination of four HCV genotypes and their subtypes. Using this assay, 61 PCR-positive Brazilian HCV sera were typed. Of the sera, 33 % had a type 1 HCV infection, 38% had type 1b (related to HCV-J), 1.5% had type 2a (related to HC-J6), 24.5 % had type 3 (related to E-b1 and HCV-T), and 3 % of the sera were co-infected. This assay format was further evaluated using 13 sera from Belgium and the Netherlands, and all of these could be classified. Two pools of Japanese sera were classified as either type 2a or were co-infected with types 1b and 2a, but no type 2b sequences were detected. Another eight PCR-positive sera were obtained from Burundi and Gabon. The sequence of the 5' untranslated region of these African viruses was strongly divergent from the three previously described types. Therefore, these isolates were tentatively classified as type 4. These and some of the other non-type 1 sera often demonstrated weaker reactivities than type 1 isolates in currently used second generation antibody confirmation assays.