Stereological analysis of Ca2+/calmodulin-dependent protein kinase IIα-containing dorsal root ganglion neurons in the rat:: Colocalization with isolectin Griffonia simplicifolia, calcitonin gene-related peptide, or vanilloid receptor 1
Stereological analysis of Ca2+/calmodulin-dependent protein kinase IIα-containing dorsal root ganglion neurons in the rat:: Colocalization with isolectin Griffonia simplicifolia, calcitonin gene-related peptide, or vanilloid receptor 1
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DOI：
10.1002/cne.10250
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发表时间：
2002-06-17
影响因子：
2.5
通讯作者：
Hargett, GL
中科院分区：
文献类型：
--
作者：
Carlton, SM;Hargett, GL
Ca 2 +/钙调蛋白依赖性蛋白激酶II（CaMKII）广泛分布于神经系统。先前的报告描述了背根神经节（DRG）神经元中CaMK Ⅱ α的免疫染色。在这项研究中，CaMKIIα在大鼠中与伤害性DRG神经元的三种假定标记物共定位，即：异凝集素（I-B4），识别小直径的“肽缺乏”神经元;降钙素基因相关肽（CGRP），识别“肽丰富”神经元;或香草素受体1（VR 1），识别由热、酸和辣椒素激活的神经元。使用免疫荧光或凝集素结合组织化学标记腰椎4和5 DRG切片，并测定单标记和双标记CaMKIIα神经元的百分比。L4 DRG中神经元总数的体视学估计值为13，815 ± 2，798，L5 DRG中为14，111 ± 4，043。单标记L4 DRG神经元的体积为41% ± 2% CaMKIIα、38% ± 3% I-B4、44% ± 3% CGRP和32% ± 6% VR 1。单标记L5 DRG神经元的体积为44% ± 5% CaMKIIα、48% ± 2% I-B4、41% ± 7% CGRP和39% ± 14% VR 1。对于L4和L5，双标记CaMKIIα神经元的估计值分别显示I-B4标记为34% ± 2%和38% ± 17%，CGRP标记为25% ± 14%和19% ± 10%，VR 1标记为37% ± 7%和38% ± 5%。相反，对于L4和L5，分别有39% ± 14%和38% ± 7%的I-B4结合神经元、24% ± 12%和23% ± 10%的CGRP神经元以及42% ± 7%和35% ± 7%的VR 1神经元标记为CaMK II α。CaMKIIα标记神经元的平均直径约为27 μm，证实该酶优先定位于小DRG神经元。这些结果提示，含有CaMK Ⅱ α的DRG神经元可能参与伤害性信息的加工。因此，这种酶可能在初级感觉神经元的伤害感受器活性和可塑性的调节中起关键作用。J. Comp.神经元448：102-110，2002.© 2002 Wiley利斯公司
The enzyme Ca2+/calmodulin‐dependent protein kinase II (CaMKII) is widely distributed in the nervous system. A previous report describes immunostaining for CaMKIIα in dorsal root ganglion (DRG) neurons. In this study, CaMKIIα is colocalized in the rat with three putative markers of nociceptive DRG neurons, isolectinGriffonia simplicifolia(I‐B4), identifying small‐diameter, “peptide‐poor” neurons; calcitonin gene‐related peptide (CGRP), identifying “ peptide‐rich” neurons; or the vanilloid receptor 1 (VR1), identifying neurons activated by heat, acid, and capsaicin. Lumbar 4 and 5 DRG sections were labeled using immunofluorescence or lectin binding histochemistry, and percentages of single and double‐labeled CaMKIIα neurons were determined. Stereological estimates of total neuron number in the L4 DRG were 13,815 ± 2,798 and in the L5 DRG were 14,111 ± 4,043. Percentages of single‐labeled L4 DRG neurons were 41% ± 2% CaMKIIα, 38% ± 3% I‐B4, 44% ± 3% CGRP, and 32% ± 6% VR1. Percentages of single‐labeled L5 DRG neurons were 44% ± 5% CaMKIIα, 48% ± 2% I‐B4, 41% ± 7% CGRP, and 39% ± 14% VR1. For L4 and L5, respectively, estimates of double‐labeled CaMKIIα neurons showed 34% ± 2% and 38% ± 17% labeled for I‐B4, 25% ± 14% and 19% ± 10% labeled for CGRP, and 37% ± 7% and 38% ± 5% labeled for VR1. Conversely, for L4 and L5, respectively, 39% ± 14% and 38% ± 7% I‐B4 binding neurons, 24% ± 12% and 23% ± 10% CGRP neurons, and 42% ± 7% and 35% ± 7% VR1 neurons labeled for CaMKIIα. The mean diameter of CaMKIIα‐ labeled neurons was approximately 27 μm, confirming that this enzyme was preferentially localized in small DRG neurons. The results indicate that subpopulations of DRG neurons containing CaMKIIα are likely to be involved in the processing of nociceptive information. Thus, this enzyme may play a critical role in the modulation of nociceptor activity and plasticity of primary sensory neurons. J. Comp. Neurol. 448:102–110, 2002. © 2002 Wiley‐Liss, Inc.