FLOW EFFECTS ON PROSTACYCLIN PRODUCTION BY CULTURED HUMAN-ENDOTHELIAL CELLS
FLOW EFFECTS ON PROSTACYCLIN PRODUCTION BY CULTURED HUMAN-ENDOTHELIAL CELLS
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DOI：
10.1126/science.3883488
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
IVES, CL
中科院分区：
文献类型：
--
作者：
FRANGOS, JA;ESKIN, SG;IVES, CL
内皮细胞功能，如花生四烯酸代谢，可以通过血流诱导的膜应力来调节。测定了原代人内皮细胞培养物在脉动流和稳态流剪切应力下前列环素的产生。流动的开始导致前列环素的生产突然增加，在几分钟内下降到一个稳定的速度。受到脉动剪切应力的细胞的稳态生产率是暴露于稳定剪切应力的细胞的两倍以上，16倍以上，比静止培养的细胞。
Endothelial cell functions, such as arachidonic acid metabolism, may be modulated by membrane stresses induced by blood flow. The production of prostacyclin by primary human endothelial cell cultures subjected to pulsatile and steady flow shear stress was measured. The onset of flow led to a sudden increase in prostacyclin production, which decreased to a steady rate within several min. The steady-state production rate of cells subjected to pulsatile shear stress was more than twice that of cells exposed to steady shear stress and 16 times greater than that of cells in stationary culture.