Celecoxib But Not Rofecoxib Inhibits the Growth of Transformed Cells in Vitro
Celecoxib But Not Rofecoxib Inhibits the Growth of Transformed Cells in Vitro
复制标题
塞来昔布而非罗非考昔在体外抑制转化细胞的生长
DOI：
10.1158/1078-0432.ccr-0412-3
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发表时间：
2004
影响因子：
11.5
通讯作者：
N. Arber
中科院分区：
文献类型：
--
作者：
D. Kazanov;H. Dvory‐Sobol;M. Pick;E. Liberman;L. Strier;Efrat Choen;V. Deutsch;T. Kunik;N. Arber
Purpose: Nonsteroidal anti-inflammatory drugs reduce the risk of colorectal cancer. The cyclooxygenase (COX) pathway of arachidonic acid metabolism is an important target for nonsteroidal anti-inflammatory drugs. Increased expression of COX-2 was recently shown to be an important step in the multistep process of colorectal cancer carcinogenesis. The new COX-2-specific inhibitors offer the benefit of cancer protection without the gastrointestinal toxicity reported for the old drugs. The purpose of this study was to compare the growth effects of two specific COX-2 inhibitors, celecoxib (Pfizer, Inc., New York, NY), and rofecoxib (Merck, White House Station, NJ) in normal and transformed enterocytes. Experimental Design: Cultures of normal rat intestinal epithelial cell line, IEC-18, vector control cells, c-K-ras, c-K-ras-bak, and antisense-bak derivatives were treated with different dosages of celecoxib (0–60 μm) and rofecoxib (0–20 μm). Cell cycle analysis and apoptosis were assessed by fluorescence-activated cell sorting analysis. Protein expression was assessed by Western blot analysis and caspases 3 and 8 activities by ELISA. Results: Celecoxib inhibited cell growth and induced apoptosis in a time- and dose-dependent manner. IEC18 parental cells were two to four times more resistant to celecoxib than ras, ras-bak, and antisense bak transformed cells that overexpress the COX-2 protein. The induction of apoptosis by celecoxib involved the caspase pathways. Rofecoxib, up to its maximal concentration of 20 μm, did not inhibit cell growth or induce apoptosis. Conclusions: Celecoxib may prove to be a very efficient component in the prevention and treatment of gastrointestinal tumors because it inhibits the growth of cancerous cells without affecting the growth of normal cells.
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DOI：
--
发表时间：
2001
期刊：
--
影响因子：
--
作者：
M. Oshima;Naomi Murai Hata;S. Kargman;Meztli Arguello;P. Luk;E. Kwong;M. Taketo;Jilly F. Evans
通讯作者：
M. Oshima;Naomi Murai Hata;S. Kargman;Meztli Arguello;P. Luk;E. Kwong;M. Taketo;Jilly F. Evans
影响因子：
11.2
作者：
C. Waskewich;R. Blumenthal;Honglan Li;R. Stein;D. Goldenberg;J. Burton
通讯作者：
C. Waskewich;R. Blumenthal;Honglan Li;R. Stein;D. Goldenberg;J. Burton
影响因子：
158.5
作者：
Steinbach, G;Lynch, PM;Kelloff, G
通讯作者：
Kelloff, G
影响因子：
3.7
作者：
DuBois, RN;Giardiello, FM;Smalley, WE
通讯作者：
Smalley, WE