Protein adsorption on colloidal alumina particles functionalized with amino, carboxyl, sulfonate and phosphate groups
Protein adsorption on colloidal alumina particles functionalized with amino, carboxyl, sulfonate and phosphate groups
复制标题
DOI：
10.1016/j.actbio.2011.09.014
复制
发表时间：
2012-03-01
影响因子：
9.7
通讯作者：
Rezwan, Kurosch
中科院分区：
文献类型：
--
作者：
Meder, Fabian;Daberkow, Timo;Rezwan, Kurosch
生物医学或生物技术应用中的胶体氧化物颗粒立即被生物介质的蛋白质包覆，这是一个受到颗粒表面特性强烈影响的过程。表面特性和蛋白质吸附之间的基本相关性，到目前为止主要是不可控的，仍然不清楚。在胶体氧化铝颗粒（d（50）= 179 +/- 8 nm）表面引入NH_2、COOH、SO_3H和PO_3H_2等功能基团，研究其对牛血清白蛋白（BSA）、溶菌酶（LSZ）和胰蛋白酶（TRY）3种模型蛋白吸附的影响。表面功能化的特征在于和详细讨论的形态，等电点，zeta电位，亲水/疏水性能，官能团密度和稳定性。然后通过评估吸附的蛋白质的量和蛋白质-颗粒缀合物的ζ电位来评估蛋白质-颗粒相互作用。蛋白质的吸附被认为是由官能团的类型，以及在给定的实验条件下预期的静电力的影响。因此，蛋白质吸附的水平可能受到表面功能化类型的具体控制。考虑到从蛋白质数据库文件计算的蛋白质的空间表面电位分布，BSA，LSZ和TRY的颗粒上的可能的吸附模式建议。提出的颗粒提供了一个很好的先决条件，为进一步调查的基本粒子-蛋白质相互作用和设计的功能梯度材料的生物医学和生物技术的应用，例如作为药物载体或蛋白质纯化。(C)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Colloidal oxide particles in biomedical or biotechnological applications immediately become coated with proteins of the biological medium, a process which is strongly influenced by the surface characteristics of the particles. Fundamental correlations between surface characteristics and the, so far mainly uncontrollable, protein adsorption are still not clear. In this study the surface of colloidal alumina particles (d(50) = 179 +/- 8 nm) was systematically adjusted with NH2, COOH, SO3H and PO3H2 functional groups to investigate the influence on the adsorption of the three model proteins, bovine serum albumin (BSA), lysozyme (LSZ) and trypsin (TRY). The surface functionalization is characterized and discussed in detail with regard to the morphology, isoelectric point, zeta potential, hydrophilic/hydrophobic properties, functional group density and stability. Protein-particle interaction was then assessed by evaluating the amount of protein adsorbed and the zeta potentials of protein-particle conjugates. Protein adsorption was found to be influenced by the type of functional group as well as the expected electrostatic forces under the given experimental conditions. The level of protein adsorption might, hence, be specifically controlled by the type of surface functionalization. Possible adsorption modes of BSA, LSZ and TRY on the particles are suggested by considering the spatial surface potential distribution of the proteins calculated from the protein database file. The particles presented provide an excellent prerequisite for further investigation of fundamental particle-protein interactions and the design of functionally graded materials for biomedical and biotechnological applications, e.g. as drug carriers or for protein purification. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.