Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research.
Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research.
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DOI：
10.1097/mco.0b013e32833cf3bc
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发表时间：
2010-09
影响因子：
3.1
通讯作者：
Kuchel GA
中科院分区：
文献类型：
--
作者：
Zhou X;Fragala MS;McElhaney JE;Kuchel GA
为临床研究人员提供对希望将细胞因子和炎症标志物测量添加到他们的研究中时需要考虑的因素的了解。炎症涉及免疫系统对组织损伤的复杂而协调的反应。在缺乏常规评估活体组织内炎症的工具的情况下，对细胞因子和其他炎症介质或标记物等体液因素的测量可以提供预测性临床信息以及对疾病机制的洞察。在历史上，酶联免疫吸附分析(ELISA)成为金标准，然而这种测量每个样本中单一蛋白质的方法限制了从有限数量的人体样本中获得的信息量。近年来，商业上可用的在有限数量中检测大量蛋白质的多重技术为研究人员提供了开始解决炎症反应复杂性的机会。然而，在研究设计、样本收集、样本测量和数据分析等方面仍需高度重视。在使用经验仍然有限的技术时，这些考虑因素尤其重要。虽然外周炎症标志物水平的测量可以为人类主题研究增加重要的机械因素，但仔细关注概念和方法方面的考虑是必不可少的，特别是在使用新技术时。
To provide clinical investigators with an understanding of factors to consider when wishing to add cytokine and inflammatory marker measurements to their studies. Inflammation involves complex and coordinated responses of the immune system to tissue damage. In the absence of tools to routinely assess inflammation within living tissues, measurements of humoral factors such as cytokines and other inflammatory mediators or markers can provide predictive clinical information plus insights into disease mechanisms. Historically, enzyme-linked immunosorbent assays (ELISAs) became the gold standard, yet this approach of measuring a single protein in each sample limits the amount of information which can be obtained from limited amounts of human sample. In recent years, commercially available multiplex technologies which detect large numbers of proteins in a limited volume have provided investigators with opportunities to begin addressing the complexity of inflammatory responses. Nevertheless, great attention needs to be paid to many aspects of study design, sample collection, sample measurement and data analysis. These considerations are especially significant when using technologies for which experience remains limited. While measurements of peripheral levels of inflammatory markers can add important mechanistic elements to human subject research, careful attention to conceptual and methodological considerations is essential, especially when using novel technologies.