Functional glucocorticoid receptor expression is required for cAMP-mediated apoptosis in a human leukemic T cell line.
Functional glucocorticoid receptor expression is required for cAMP-mediated apoptosis in a human leukemic T cell line.
复制标题
功能性糖皮质激素受体表达是人类白血病 T 细胞系中 cAMP 介导的细胞凋亡所必需的。
作者：
J. Kiefer;S. Okret;M. Jondal;D. McConkey
研究了糖皮质激素受体（GR）参与cAMP诱导的CEM. C7人T-ALL细胞系GR缺陷衍生物的细胞凋亡。孵育的亲本CEM.C7细胞与提高cAMP水平的试剂（二丁酰cAMP和毛喉素）导致凋亡性细胞死亡的DNA片段化特征，而GR缺陷的ICR. 27细胞对cAMP的细胞溶解作用不敏感。通过转染重建GR表达不仅恢复了糖皮质激素对ICR. 27细胞的敏感性，而且还促进了对cAMP诱导凋亡的敏感性。因此，cAMP诱导的T细胞凋亡似乎通过糖皮质激素受体功能的至少某些方面的配体非依赖性刺激而发生。
The involvement of the glucocorticoid receptor (GR) in cAMP-induced apoptosis in a GR-deficient derivative of the CEM.C7 human T-ALL line was investigated. Incubation of the parental CEM.C7 cells with agents that elevate cAMP levels (dibutyryl cAMP and forskolin) resulted in DNA fragmentation characteristic of apoptotic cell death, whereas the GR-deficient ICR.27 cells were insensitive to the cytolytic effects of cAMP. Reconstitution of GR expression by transfection not only restored glucocorticoid sensitivity to the ICR.27 cells, but also promoted sensitivity to induction of apoptosis by cAMP. Thus, cAMP-induced apoptosis in T cells appears to occur via ligand-independent stimulation of at least some aspects of glucocorticoid receptor function.