Noninvasive and early diagnosis of acquired brain injury using fluorescence imaging in the NIR-II window.
Noninvasive and early diagnosis of acquired brain injury using fluorescence imaging in the NIR-II window.
复制标题
使用 NIR-II 窗口中的荧光成像对获得性脑损伤进行无创早期诊断。
DOI：
10.1364/boe.442657
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发表时间：
2021
影响因子：
3.4
通讯作者：
Bobo Gu
中科院分区：
文献类型：
--
作者：
Xinyan Jiang;Rui Pu;Cheng Wang;Jiale Xu;Y. Tang;Shuhong Qi;Qiuqiang Zhan;Xunbin Wei;Bobo Gu
获得性脑损伤（ABI）是所有脑损伤的统称，是最危险的疾病之一，具有较高的发病率和死亡率，因此ABI的早期诊断极为重要。目前的诊断方法主要包括X射线计算机断层扫描和磁共振血管造影，但在诊断ABI时，这些方法存在空间分辨率有限和扫描时间长等局限性。在此，我们报道了一种无需开颅即可对小鼠脑血管进行透颅荧光成像的方法，该方法利用了1.3 - 1.7μm近红外窗口（近红外二区窗口）下转换纳米粒子（DCNPs）的荧光特性。近红外二区荧光成像方法具有22.79μm的高空间分辨率，能够在小鼠遭受刺伤（创伤性脑损伤）和缺血性中风（非创伤性脑损伤）后，快速区分其脑损伤区域，使其成为ABI无创早期诊断的有力工具。
Acquired brain injury (ABI), which is the umbrella term for all brain injuries, is one of the most dangerous diseases resulting in high morbidity and mortality, making it extremely significant to early diagnosis of ABI. Current methods, which are mainly composed of X-ray computed tomography and magnetic resonance angiography, remain limited in diagnosis of ABI with respect to limited spatial resolution and long scanning times. Here, we reported through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy, utilizing the fluorescence of down-conversion nanoparticles (DCNPs) in the 1.3 - 1.7 μm near-infrared window (NIR-II window). Due to its high spatial resolution of 22.79 μm, the NIR-II fluorescence imaging method could quickly distinguish the brain injury region of mice after performing the stab wound injury (traumatic brain injury) and ischemic stroke (non-traumatic brain injury), enabling it a powerful tool in the noninvasive and early diagnosis of ABI.