Acidic mucin layer facilitates micelle dissociation and fatty acid diffusion.
Acidic mucin layer facilitates micelle dissociation and fatty acid diffusion.
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酸性粘蛋白层促进胶束解离和脂肪酸扩散。
DOI：
10.1152/ajpgi.1990.259.4.g671
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Reed,MA
中科院分区：
文献类型：
--
作者：
Shiau,YF;Kelemen,RJ;Reed,MA
肠上皮表面粘蛋白层的存在被认为是维持酸性微气候的重要因素。这种低pH隔室的存在已被证明有利于脂肪酸摄取。在纯化的酸性粘蛋白层中研究了导致脂肪酸摄取增强的机制。我们的研究结果表明，一个低pH值的隔间的存在下，确实有利于牛磺胆酸和油酸的混合胶束的解离。释放的脂肪酸在粘蛋白层形成乳液，并且该事件可以通过肉眼观察到。当通过光子相关光谱法检查胶束溶液中的颗粒的尺寸时，发现单独的酸化可以导致形成尺寸显著大于胶束的颗粒。随着标记的脂肪酸的使用，光密度的变化可以与粘蛋白层中出现的脂肪酸的量以渐近的方式相关，这表明使用浊度作为指标可能低估脂肪酸扩散。尽管有此限制，粘蛋白层中的脂肪酸扩散速率估计为缓冲溶液中的400%。
The presence of a mucin layer on the surface of the intestinal epithelium has been suggested as an important factor in maintaining an acidic microclimate. The presence of such a low-pH compartment has been shown to facilitate fatty acid uptake. The mechanisms leading to the enhancement of fatty acid uptake were investigated in a purified acidic mucin layer. Our results indicate that the presence of a low-pH compartment indeed facilitates the dissociation of mixed micelles made of taurocholate and oleic acid. The released fatty acid formed an emulsion at the mucin layer, and this event could be visualized by the naked eye. When the size of the particles in the micelle solution was examined by photon correlation spectroscopy, it was found that acidification alone can lead to the formation of particles with size substantially greater than that of micelles. With the use of labeled fatty acid, the change in optical density can be correlated to the amount of fatty acid appearing in the mucin layer in an asymptotic fashion, suggesting that using the turbidity as an indicator might underestimate fatty acid diffusion. Despite this limitation, the rate of fatty acid diffusion in the mucin layer was estimated to be 400% of that in the buffer solution.