IDENTIFICATION OF A SOLUBLE SALICYLIC ACID-BINDING PROTEIN THAT MAY FUNCTION IN SIGNAL TRANSDUCTION IN THE PLANT DISEASE-RESISTANCE RESPONSE
IDENTIFICATION OF A SOLUBLE SALICYLIC ACID-BINDING PROTEIN THAT MAY FUNCTION IN SIGNAL TRANSDUCTION IN THE PLANT DISEASE-RESISTANCE RESPONSE
复制标题
DOI：
10.1073/pnas.88.18.8179
复制
发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
KLESSIG, DF
中科院分区：
文献类型：
--
作者：
CHEN, ZX;KLESSIG, DF
最近研究表明，水杨酸（SA）可能作为诱导烟草和黄瓜系统获得抗性的内源信号分子。此外，SA是一些产热植物花序中产生热量和气味的内源调节剂。然而，目前还没有关于SA在植物信号转导中的作用方式的信息。在寻找可能的细胞因子，直接与SA相互作用，我们已经检测到并部分表征了烟草叶片中的SA结合蛋白。SA结合活性是SDS和蛋白酶敏感的，并且表现为具有650 kDa表观质量的可溶性蛋白。该蛋白对SA具有14 μ M的表观K（d），这与观察到的诱导植物抗性反应的SA的生理浓度范围一致。此外，SA类似物与SA竞争结合该可溶性蛋白的能力与它们诱导与抗病性相关的基因表达的生物活性严格相关。生物活性类似物有效地抑制SA结合，而生物非活性类似物则不能。这些结果共同表明，这种SA结合蛋白可能在感知和转导SA信号到适当的响应元件，最终激活一个或多个植物抗病反应中发挥作用。
It has recently been demonstrated that salicylic acid (SA) may serve as an endogenous signal molecule in the induction of systemic acquired resistance in tobacco and cucumber. In addition, SA is an endogenous regulator of heat and odor production in the inflorescence of some thermogenic plants. No information, however, is currently available concerning the mode(s) of action of SA in plant signal transduction. In a search for possible cellular factors that directly interact with SA, we have detected and partially characterized a SA-binding protein in tobacco leaves. The SA-binding activity is both SDS and proteinase sensitive and behaves as a soluble protein with an apparent mass of 650 kDa. The protein has an apparent K(d) of 14-mu-M for SA, which is consistent with the range of physiological concentrations of SA observed for the induction of plant resistance responses. Furthermore, the ability of SA analogues to compete with SA for binding to this soluble protein is strictly correlated with their biological activity to induce the expression of genes associated with disease resistance. Biologically active analogues effectively inhibit SA binding while biologically inactive analogues do not. These results collectively indicate that this SA-binding protein may play a role in perceiving and transducing the SA signal to appropriate response elements, which ultimately activate one or more of the plant disease-resistance responses.