Bladder squamous cell carcinomas express psoriasin and externalize it to the urine
Bladder squamous cell carcinomas express psoriasin and externalize it to the urine
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DOI：
10.1016/s0022-5347(01)66118-4
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发表时间：
1996-06-01
影响因子：
6.6
通讯作者：
Orntoft, TF
中科院分区：
文献类型：
--
作者：
Celis, JE;Rasmussen, HH;Orntoft, TF
目的：报告一个单一的生物标志物，psoriasin（Mr 11.0 kd，pI 6.2），钙结合蛋白，主要是由复层鳞状上皮细胞表达，并外化到膀胱鳞状细胞癌（SCC）轴承patients.Materials和方法：蛋白质表达谱SCC术后立即获得的双向凝胶电泳和考马斯蓝染色进行了分析。通过微测序和免疫印迹确定蛋白质身份。结果：从100例疑似移行细胞癌（TCC）患者的标本中鉴定出4例SCC。4个SCC（56-1，III级，T4; 181-1，I级，T3; 219-1，III级，T3和239-1，未确定的等级，T2-4）的蛋白质谱与角质形成细胞的蛋白质谱相似，表明这些细胞表达基因表达的早期发育模式。除了表达角质化复层鳞状上皮细胞的特征性标志物外，SCC还表现出银屑病蛋白，一种由角质形成细胞外化到培养基中的蛋白。免疫组化结果显示，3例阳性细胞主要分布于鳞状珠内。二维凝胶免疫印迹法证实了4名SCC患者尿液中存在银屑病。对43例非SCC膀胱肿瘤患者的尿液进行类似分析，发现7例阳性，其中一些可能反映了鳞状分化。对13名对照个体（12名年龄匹配的男性和1名42岁的女性）的尿液分析显示2例阳性。免疫印迹法的SCC患者的血清蛋白与银屑病抗体未能揭示protein.Conclusion：结果指向银屑病，单独或作为生物标志物的一部分，作为一个潜在的标记物的SCC患者的非侵入性随访。
Purpose: To report a single biomarker, psoriasin (Mr 11.0 kd, pI 6.2), a calcium binding protein which is expressed largely by stratified squamous epithelia and is externalized to the urine of bladder squamous cell carcinoma (SCC) bearing patients.Materials and Methods: Protein expression profiles of SCCs obtained immediately after surgery were analyzed by two-dimensional gel electrophoresis and Coomassie blue staining. Protein identity was determined by microsequencing and immunoblotting. Protein expression in cryosections was studied by immunofluorescence.Results: Four patients with SCC were identified from 100 samples of patients with suspected transitional cell carcinoma (TCC). The protein profiles of the 4 SCCs (56-1, grade III, T4; 181-1, grade I, T3; 219-1, grade III, T3 and 239-1, grade not determined, T2-4) resembled that of keratinocytes, suggesting that these cells express an early developmental pattern of gene expression. Besides expressing markers characteristic of keratinizing stratified squamous epithelia, the SCCs exhibited psoriasin, a protein externalized to the medium by keratinocytes. Immunohistochemistry of 3 of the SCCs with psoriasin antibodies showed that the positive cells were confined chiefly to the ''squamous pearls.'' The presence of psoriasin in the urine of the 4 SCC patients was demonstrated by two-dimensional gel immunoblotting. Similar analysis of 43 urines from patients with bladder tumors other than SCC revealed 7 positives, some of which may reflect squamous differentiation. Analysis of the urine of 13 control individuals (12 males matched by age and a 42-year-old female) revealed 2 positives. Immunoblotting of the SCC patients' serum proteins with psoriasin antibodies failed to reveal the protein.Conclusion: The results point towards psoriasin, alone or as part of a biomarker profile, as a potential marker for the noninvasive follow-up of patients with SCC.