Monitoring the Biodegradation of Dendritic Near-Infrared Nanoprobes by in Vivo Fluorescence Imaging
Monitoring the Biodegradation of Dendritic Near-Infrared Nanoprobes by in Vivo Fluorescence Imaging
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DOI：
10.1021/mp8000952
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
--
作者：
Almutairi, Adah;Akers, Walter J.;Frechet, Jean M. J.
合成聚合物和树状大分子已被医学界广泛应用于克服生物屏障和增强体内生物医学应用。尽管生物材料得到了广泛的应用，但通常很难在体内无创地监测它们的命运。在这里，我们报告了多层纳米探针，由近红外核心组成，纳米封装在可生物降解的树状聚合物中，并被聚乙烯氧化物外壳包围。树突支架中近红外荧光团的共价包封增强了纳米探针的稳定性，增加了抗酶氧化的能力，延长了血液停留时间。利用无创全身荧光寿命成像技术对树突状脂肪族聚酯纳米探针的生物降解过程进行了深入研究。随着树突壳的生物降解，近红外探针暴露在外，从而能够监测体内荧光寿命的变化。
Synthetic polymers and dendrimers have been widely used by the medical community to overcome biological barriers and enhance in vivo biomedical applications. Despite the widespread use of biomaterials it has been generally extremely difficult to monitor noninvasively their fate in vivo. Here we report multilayered nanoprobes, consisting of a near-infrared core, nanoencapsulated in a biodegradable dendrimer, and surrounded by a shell of polyethylene oxide. Covalent encapsulation of the near-infrared fluorophores in the dendritic scaffold conferred enhanced stability to the nanoprobe with added resistance to enzymatic oxidation and prolonged blood residence time. Insight into the time course of biodegradation of the dendritic aliphatic polyester nanoprobe was gained using noninvasive whole body in vivo fluorescence lifetime imaging. As the dendritic shell biodegrades the NIR probe becomes exposed, enabling monitoring of fluorescence lifetime changes in vivo.