CLONING AND EXPRESSION OF CDNA-ENCODING A NEW-TYPE OF ASCORBATE PEROXIDASE FROM SPINACH
CLONING AND EXPRESSION OF CDNA-ENCODING A NEW-TYPE OF ASCORBATE PEROXIDASE FROM SPINACH
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DOI：
10.1016/0014-5793(95)00539-l
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发表时间：
1995-06-19
期刊：
影响因子：
3.5
通讯作者：
SHIGEOKA, S
中科院分区：
文献类型：
--
作者：
ISHIKAWA, T;SAKAI, K;SHIGEOKA, S
使用抗眼虫抗坏血酸过氧化物酶的单克隆抗体从菠菜 cDNA 文库中分离出编码过氧化物酶的 cDNA 克隆 (SAP1)。推导的 SAP1 氨基酸序列与植物来源的胞质抗坏血酸过氧化物酶的同源性高于与细菌过氧化物酶和经典植物过氧化物酶的同源性。在大肠杆菌中表达的重组 SAP1 蛋白的过氧化物酶活性，抗坏血酸比愈创木酚高 1.6 倍，与内源性胞质抗坏血酸过氧化物酶的活性相似。在此我得出结论，SAP1属于菠菜中的一种新型抗坏血酸过氧化物酶。
A cDNA clone (SAP1) encoding a peroxidase was isolated from a spinach cDNA library using monoclonal antibodies raised against Euglena ascorbate peroxidase. The deduced amino acid sequence of SAP1 had higher homology with the cytosolic ascorbate peroxidases from plant sources than with bacterial peroxidases and classical plant peroxidases. The peroxidase activity of recombinant SAP1 protein expressed in E. coli was 1.6-fold higher with ascorbate than with guaiacol, which was similar to those of endogenous cytosolic ascorbate peroxidases. Here me conclude that SAP1 belongs to a new type of ascorbate peroxidase from spinach.