Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi).
Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi).
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金羚牛 (Budorcas Taxicolor bedfordi) 肠道微生物群的特征
DOI：
10.1186/s13568-017-0374-5
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发表时间：
2017-12
期刊：
影响因子：
3.7
通讯作者：
Tang X
中科院分区：
文献类型：
--
作者：
Chen J;Zhang H;Wu X;Shang S;Yan J;Chen Y;Zhang H;Tang X
哺乳动物的肠道菌群是一个复杂的生态系统，对维持肠道内稳态和宿主健康至关重要。高通量测序使我们能够更深入地了解细菌的结构和多样性。为了改善濒临灭绝的扭角羚的健康状况，我们首先利用16 S rRNA基因V3-V4高变区的高通量测序来表征健康扭角羚的粪便微生物群。结果表明：①肠道菌群由21门40纲62目96科216属组成。厚壁菌门（67.59%）是最丰富的门，其次是拟杆菌门（23.57%）和变形菌门（2.37%）。春季扭角羚的丰富度高于冬季，但群落多样性和均匀度差异不显著。第三，四只雌性扭角羚的微生物群高度相似，五只雄性扭角羚的微生物群相对高度相似。这些结果表明，季节和性别对扭角羚粪便菌群有一定的影响。这一发现为扭角羚肠道菌群的研究提供了理论依据，并可能为保护这一濒危物种提供新的见解。
The gut microbiota of mammals is a complex ecosystem, which is essential for maintaining gut homeostasis and the host’s health. The high throughput sequencing allowed us to gain a deeper insight into the bacterial structure and diversity. In order to improve the health status of the endangered golden takins, we first characterized the fecal microbiota of healthy golden takins using high throughput sequencing of the 16S rRNA genes V3–V4 hypervariable regions. Our results showed that, Firstly, the gut microbiota community comprised 21 phyla, 40 classes, 62 orders, 96 families, and 216 genera. Firmicutes (67.59%) was the most abundant phylum, followed by Bacteroidetes (23.57%) and Proteobacteria (2.37%). Secondly, the golden takin maintained higher richness in spring than in the winter while community diversity and evenness was not significantly different. Thirdly, four female golden takins demonstrated highly similar microbiota and the five golden takin males had relatively highly similar microbiota. All of our results might indicate that the fecal microbiota of golden takins were influenced by the season and the animal’s sex. The findings provided theoretical basis regarding the gut microbiota of golden takins and may offer new insights to protect this endangered species.