Major polypeptide of duck hepatitis B surface antigen particles.
Major polypeptide of duck hepatitis B surface antigen particles.
复制标题
鸭乙型肝炎表面抗原颗粒的主要多肽。
DOI：
10.1128/jvi.48.2.534-541.1983
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发表时间：
1983
影响因子：
5.4
通讯作者：
Robinson,WS
中科院分区：
文献类型：
--
作者：
Marion,PL;Knight,SS;Feitelson,MA;Oshiro,LS;Robinson,WS
本文报道了用高速区带离心和等密度离心法从感染鸭B肝炎病毒的北京家鸭血清中分离到的40 ~ 50 nm的多形性颗粒。对这些被称为鸭乙型肝炎B表面抗原颗粒的颗粒进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳多肽分析，发现主要成分是一个单一的17，500-道尔顿多肽。这一结果与相关哺乳动物病毒（包括B型肝炎病毒）表面抗原的多肽分析相反，在该分析中，观察到分子量范围为23，000至29，000的主要多肽双联体。17，500-道尔顿多肽的胰蛋白酶图谱与B型肝炎表面抗原adw亚型的主要非糖基化多肽相似。还描述了纯化鸭病毒颗粒抗体的血清学测定。
The 40- to 50-nm pleomorphic particles found in the sera of domestic Pekin ducks infected with duck hepatitis B virus were purified by rate zonal and isopycnic centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic polypeptide analysis of these particles, called duck hepatitis B surface antigen particles, revealed the major component to be a single 17,500-dalton polypeptide. This result is in contrast to polypeptide analyses of the surface antigens of related mammalian viruses, including hepatitis B, in which a major doublet of polypeptides is seen with molecular weights ranging from 23,000 to 29,000. Tryptic maps of 17,500-dalton polypeptide resembled that of the major non-glycosylated polypeptide of the adw subtype of hepatitis B surface antigen. A serological assay for antibody to the purified duck virus particles is also described.