Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery
Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery
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DOI：
10.1016/j.carbpol.2016.08.046
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发表时间：
2017-01-02
影响因子：
11.2
通讯作者：
Abnous, Khalil
中科院分区：
文献类型：
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作者：
Alibolandi, Mona;Mohammadi, Marzieh;Abnous, Khalil
在当前的研究中，葡聚糖（DEX）共价缀合到纳米GO片的表面，从而制备稳定的生物相容性葡聚糖涂覆的GO（GO-DEX）。所制备的GO-DEX在高达300 μ g/mL的浓度下对4 T1乳腺癌细胞系是无毒的。AS 1411适体，一种可以通过核仁素识别来提高细胞内摄取的ssDNA适体，也已经被引入GO-DEX中的DEX的羟基以产生GO-DEX-Apt。此外，姜黄素（CUR），一种天然多酚，发现在姜黄（姜黄）的根茎中，显示出抑制作用，负载到GO-DEX和GO-DEX-Apt通过pi-pi堆积相互作用与高负载能力（类似于29重量%）。经荧光显微镜和流式细胞仪检测，GO-DEX-Apt-CUR能有效进入核仁素过表达的4 T1和MCF-7细胞，并显示出明显的细胞毒活性。基于DEX涂覆的GO的这些类型的靶向纳米级药物递送载体可以在癌症化疗中找到潜在的应用。(C)2016爱思唯尔有限公司版权所有
In the current study, dextran (DEX) was covalently conjugated to the surface of nano-GO sheets, making stable biocompatible dextran coated GO (GO-DEX). The prepared GO-DEX was nontoxic to 4T1 mammary carcinoma cell line at concentrations up to 300 mu g/mL.AS1411 aptamer, a ssDNA aptamer which can improve the intracellular uptake by nucleolin recognition, also has been introduced to hydroxyl groups of DEX in GO-DEX to produce GO-DEX-Apt. Moreover, curcumin (CUR), a natural polyphenol, found in the rhizomes of Curcuma Tonga (turmeric) which shows antineoplastic effects, was loaded onto the GO-DEX and GO-DEX-Apt via pi-pi stacking interactions with a high loading capacity (similar to 29 wt%). The GO-DEX-Apt-CUR could efficiently enter into 4T1 and MCF-7 nucleolin over-expressed cancer cells confirmed by fluorescence microscope and flowcytometry, and it also showed significantly higher cytotoxicity. These types of targeted nanoscale drug delivery vehicles on the basis of DEX coated GO may find potential application in cancer chemotherapy. (C) 2016 Elsevier Ltd. All rights reserved.