Methane Production From Different Parts of Corn Stover via a Simple Co-culture of an Anaerobic Fungus and Methanogen

Methane Production From Different Parts of Corn Stover via a Simple Co-culture of an Anaerobic Fungus and Methanogen
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通过厌氧真菌和产甲烷菌的简单共培养从玉米秸秆的不同部分生产甲烷

DOI:
10.3389/fbioe.2020.00314
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发表时间:
2020-04-30
影响因子:
5.7
通讯作者:
Zhu, Weiyun
Zhu, Weiyun
中科院分区:
工程技术2区
文献类型:
--
作者:
Li, Yuqi;Hou, Zhesheng;Zhu, Weiyun

文献摘要

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为了确定提高玉米秸秆利用率的方法,本研究通过厌氧真菌(Pecoramyces)和产甲烷菌(Methanobrevibacter)的简单共培养,研究了玉米秸秆不同部分的甲烷产量。以玉米秸秆的茎髓、叶片或茎皮为底物,在39℃下培养72 h。结果表明,茎皮的消化率最低(38.0±1.36%),中性洗涤物即细胞可溶性物(31.6±0.45%),木质素含量最高(P < 0.05),为4.8±0.56%。叶片的甲烷转化率(56.6±0.76 mL/g消化底物)显著高于茎髓(49.2±1.60 mL/g消化底物),尽管它们的甲烷产量水平相似(分别为42.4±1.0 mL和40.9±1.35 mL)。在厌氧真菌和产甲烷菌的简单共培养中,玉米秸秆的叶片和茎髓都有可能产生甲烷。
To determine ways to improve the utilization of corn stover, this study investigated methane production from different parts of corn stover using a simple co-culture of an anaerobic fungus (Pecoramyces species) and methanogen (Methanobrevibacter species). The simple co-culture was incubated with the stem pith, leaf blade, or stem bark of corn stover (as substrates) at 39°C for 72 h. The results showed that the stem bark had the lowest (P < 0.05) digestibility (38.0 ± 1.36%) and neutral detergent solubles, that is, cell solubles (31.6 ± 0.45%), and the highest (P < 0.05) lignin content (4.8 ± 0.56%). The leaf blade had a significantly higher methane conversion rate (56.6 ± 0.76 mL/g digested substrate) than the stem pith (49.2 ± 1.60 mL/g digested substrate), even though they showed similar levels of methane production (42.4 ± 1.0 mL and 40.9 ± 1.35 mL, respectively). Both the leaf blade and stem pith of corn stover have the potential to produce methane in a simple co-culture of an anaerobic fungus and methanogen.