Identification of White Adipocyte Progenitor Cells In Vivo
Identification of White Adipocyte Progenitor Cells In Vivo
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DOI：
10.1016/j.cell.2008.09.036
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发表时间：
2008-10-17
期刊：
影响因子：
64.5
通讯作者：
Friedman, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Rodeheffer, Matthew S.;Birsoy, Kivanc;Friedman, Jeffrey M.
与肥胖相关的白色脂肪组织(WAT)质量增加是脂肪细胞增殖和肥大的结果。然而，控制脂肪细胞数量的机制尚不清楚，部分原因是生理脂肪细胞前体细胞在体内的身份尚未确定。在这份报告中，我们采用多种方法，包括体内脂肪块重建后的非侵入性检测，来鉴定居住在成人WAT中的早期脂肪细胞前体细胞亚群(LIN(-)：CD29(+)：CD34(+)：SCA-1(+)：CD24(+))。当被注射到A-Zip脂肪营养不良小鼠的残余脂肪垫中时，这些细胞重建了正常的Wat储存库，并挽救了在这些动物中发展起来的糖尿病表型。本报告提供了一种存在于脂肪组织基质中的未分化的脂肪细胞前体亚群的鉴定，该亚群能够在体内增殖和分化为脂肪库。
The increased white adipose tissue (WAT) mass associated with obesity is the result of both hyperplasia and hypertrophy of adipocytes. However, the mechanisms controlling adipocyte number are unknown in part because the identity of the physiological adipocyte progenitor cells has not been defined in vivo. In this report, we employ a variety of approaches, including a noninvasive assay for following fat mass reconstitution in vivo, to identify a subpopulation of early adipocyte progenitor cells (Lin(-): CD29(+): CD34(+): Sca-1(+): CD24(+)) resident in adult WAT. When injected into the residual fat pads of A-Zip lipodystrophic mice, these cells reconstitute a normal WAT depot and rescue the diabetic phenotype that develops in these animals. This report provides the identification of an undifferentiated adipocyte precursor subpopulation resident within the adipose tissue stroma that is capable of proliferating and differentiating into an adipose depot in vivo.