Applications of the second virial coefficient: protein crystallization and solubility.
Applications of the second virial coefficient: protein crystallization and solubility.
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DOI：
10.1107/s2053230x1400867x
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Delucas LJ
中科院分区：
文献类型：
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作者：
Wilson WW;Delucas LJ
本文重点介绍了一些地面研究产生的NASA的微重力蛋白质晶体生长（PCG）计划，并包括一个更详细的讨论的历史和进展，在一个NASA资助的PCG调查涉及使用测得的第二维里系数（B值）作为诊断指标的溶液条件有利于蛋白质结晶。本文首先强调了一些来自NASA微重力蛋白质晶体生长（PCG）计划的地面研究。其次是一个更详细的讨论的历史和进展，在美国宇航局资助的PCG调查涉及使用测得的第二维里系数（B值）作为诊断指标的溶液条件有利于蛋白质结晶。测量的B值的第二个应用涉及确定改善或最大化水和膜蛋白的溶解度的溶液条件。这两个重要的应用导致了几项技术改进，简化了所需的实验专业知识，使膜蛋白的测量和提高诊断能力和测量通量。
This article highlights some of the ground-based studies emanating from NASA’s Microgravity Protein Crystal Growth (PCG) program, and includes a more detailed discussion of the history and the progress made in one of the NASA-funded PCG investigations involving the use of measured second virial coefficients (B values) as a diagnostic indicator of solution conditions conducive to protein crystallization. This article begins by highlighting some of the ground-based studies emanating from NASA’s Microgravity Protein Crystal Growth (PCG) program. This is followed by a more detailed discussion of the history of and the progress made in one of the NASA-funded PCG investigations involving the use of measured second virial coefficients (B values) as a diagnostic indicator of solution conditions conducive to protein crystallization. A second application of measured B values involves the determination of solution conditions that improve or maximize the solubility of aqueous and membrane proteins. These two important applications have led to several technological improvements that simplify the experimental expertise required, enable the measurement of membrane proteins and improve the diagnostic capability and measurement throughput.