Diversity of bacterial Populations on the tongue dorsa of patients with halitosis and healthy patients
Diversity of bacterial Populations on the tongue dorsa of patients with halitosis and healthy patients
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DOI：
10.1128/jcm.41.2.558-563.2003
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发表时间：
2003-02-01
影响因子：
9.4
通讯作者：
Paster, BJ
中科院分区：
文献类型：
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作者：
Kazor, CE;Mitchell, PM;Paster, BJ
本研究的主要目的是利用培养不依赖的分子方法比较健康者和口臭者舌背的微生物特征。我们的总体目标是确定舌背表面的细菌多样性，这是我们正在努力确定所有可培养和未培养的口腔物种的一部分。对没有口臭症状的健康受试者和有口臭的受试者的舌背刮痕进行分析，口臭的定义是感官评分为2分或以上，挥发性硫化合物水平大于200 ppb。用普遍保守的引物PCR扩增舌背刮痕DNA中的16S rRNA基因，并将其克隆到大肠杆菌中。一般来说，每个受试者的克隆数为50到100个。对从健康人舌背分离的51株细菌进行了分析。从分离物的16S rRNA基因中克隆插入片段的大约500个碱基的部分序列与已知物种或种型的序列进行比较，以确定物种身份或最近的亲缘关系。获得了近1500个碱基的完整序列，用于潜在的新种或种型。在对大约750个克隆的分析中，鉴定出92种不同的细菌种类。大约一半的克隆被确定为种型，其中29个是舌头微生物群的新物种。92个种或种型中有51个在一个以上的受试者中检测到。与健康受试者最相关的物种是唾液链球菌、粘胶罗氏菌和一种未表征的真细菌（菌株FTB41）。唾液链球菌是健康受试者的优势菌种，占每个健康受试者所分析的总克隆的12 - 40%。总体而言，健康受试者的舌背上的优势菌群与口臭受试者的舌背上的优势菌群不同。与口臭最相关的物种是Dialister的一个种型（克隆BS095） parvulium，未栽培门TM7的一个种型（克隆DR034） ulbacterium sulci， moorei Solobacterium moorei和链球菌的一个种型（克隆BW009）。在我们不断努力获得所有可培养和未培养的口腔定植物种的完整16S rRNA序列的基础上，现在有超过600个物种。
The primary purpose of the present study was to compare the microbial profiles of the tongue dorsa of healthy subjects and subjects with halitosis by using culture-independent molecular methods. Our overall goal was to determine the bacterial diversity on the surface of the tongue dorsum as part of our ongoing efforts to identify all cultivable and not-yet-cultivated species of the oral cavity. Tongue dorsum scrapings were analyzed from healthy subjects with no complaints of halitosis and subjects with halitosis, defined as an organoleptic score of 2 or more and volatile sulfur compound levels greater than 200 ppb. 16S rRNA genes from DNA isolated from tongue dorsum scrapings were amplified by PCR with universally conserved bacterial primers and cloned into Escherichia coli. Typically, 50 to 100 clones were analyzed from each subject. Fifty-one strains isolated from the tongue dorsa of healthy subjects were also analyzed. Partial sequences of approximately 500 bases of cloned inserts from the 16S rRNA genes of isolates were compared with sequences of known species or phylotypes to determine species identity or closest relatives. Nearly complete sequences of about 1,500 bases were obtained for potentially novel species or phylotypes. In an analysis of approximately 750 clones, 92 different bacterial species were identified. About half of the clones were identified as phylotypes, of which 29 were novel to the tongue microbiota. Fifty-one of the 92 species or phylotypes were detected in more than one subject. Those species most associated with healthy subjects were Streptococcus salivarius, Rothia mucilaginosa, and an uncharacterized species of Eubacterium (strain FTB41). Streptococcus salivarius was the predominant species in healthy subjects, as it represented 12 to 40% of the total clones analyzed from each healthy subject. Overall, the predominant microbiota on the tongue dorsa of healthy subjects was different from that on the tongue dorsa of subjects with halitosis. Those species most associated with halitosis were Atopobium parvulum, a phylotype (clone BS095) of Dialister, Eubacterium sulci, a phylotype (clone DR034) of the uncultivated phylum TM7, Solobacterium moorei, and a phylotype (clone BW009) of Streptococcus. On the basis of our ongoing efforts to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species that colonize the oral cavity, there are now over 600 species.