Synthesis and bioactivity of fluorescence- and biotin-labeled lipid A analogues for investigation of recognition mechanism in innate immunity
Synthesis and bioactivity of fluorescence- and biotin-labeled lipid A analogues for investigation of recognition mechanism in innate immunity
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DOI：
10.1016/j.tetlet.2005.11.041
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发表时间：
2006-01-23
影响因子：
1.8
通讯作者：
Fukase, K
中科院分区：
文献类型：
--
作者：
Fujimoto, Y;Kimura, E;Fukase, K
合成了荧光标记和生物素标记的类脂A类似物，用于研究细菌脂多糖（LPS）/类脂A在先天免疫系统中的识别。为了引入标记部分，使用亲水性戊二酰-葡萄糖接头来维持生物活性，并且还用于防止导致荧光淬灭的自聚集。并对标记化合物的生物活性进行了观察。(c)2005爱思唯尔有限公司保留所有权利。
Fluorescence- and biotin-labeled lipid A analogues were synthesized for the investigation of bacterial lipopolysaccharide (LPS)/lipid A recognition in the innate immune system. For the introduction of the labeling moiety, a hydrophilic glutaryl-glucose linker was used for maintaining the bioactivity and also for preventing self-aggregation, which causes quenching of the fluorescence. We also observed the biological activity of the labeled compounds. (c) 2005 Elsevier Ltd. All rights reserved.