Method for making selective lesions of the hippocampus in macaque monkeys using NMDA and a longitudinal surgical approach
Method for making selective lesions of the hippocampus in macaque monkeys using NMDA and a longitudinal surgical approach
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DOI：
10.1002/hipo.10150
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发表时间：
2004-01-01
期刊：
影响因子：
3.5
通讯作者：
Murray, EA
中科院分区：
文献类型：
--
作者：
Hampton, RR;Buckmaster, CA;Murray, EA
我们描述了一种方法，用于在猕猴海马的选择性病变，使用磁共振成像（MRI）引导的立体定位方法，其中兴奋性毒素N-甲基-D-天冬氨酸（NMDA）注射间隔沿着一个单一的针轨道，纵向延伸通过rostrocaudal程度的海马。在六只恒河猴（Macaca mulatta）上进行程序，并且在对脑进行标准组织学处理后，用体内MRI（n = 3）或组织的死后显微镜检查（n = 3）进行评估。根据我们的广泛经验，标准的立体定位程序中，鹅膏蕈氨酸（IBO）是通过背侧方法注射，我们报告说，新的方法提供了一个可行的和潜在的有利的替代标准程序。首先，纵向方法结合N-甲基-D-天冬氨酸（NMDA）注射增加海马兴奋性毒性病变的可靠性和有效性，可能是通过限制注射剂泄漏到脑室。第二，与标准手术相比，本手术术后恢复更快。第三，由于新方法需要比标准方法更少的针穿透，它很可能减少海马外组织梗死的机会。最后，新的手术方法可能提供一种机制，从一个单一的插管注入剂到海马。Published（C）2003 Wiley-Liss，Inc.匕首
We describe a method for making selective lesions of the hippocampus in macaque monkeys, using a magnetic resonance imaging (MRI)-guided stereotaxic approach in which the excitotoxin N-methyl-D-aspartic acid (NMDA) is injected at intervals along a single needle track that extends longitudinally through the rostrocaudal extent of the hippocampus. Procedures were conducted on six rhesus monkeys (Macaca mulatta) and were assessed with either in vivo MRI (n = 3) or postmortem microscopic examination of the tissue after standard histological processing of the brains (n = 3). Based on our extensive experience with the standard stereotaxic procedure in which ibotenic acid (IBO) is injected via the dorsal approach, we report that the new method provides a viable and potentially advantageous alternative to the standard procedure. First, the longitudinal approach combined with N-methyl-D-aspartic acid (NMDA) injections increases the reliability and efficacy of hippocampal excitotoxic lesions, probably by limiting the leakage of injectant into the ventricles. Second, the present procedure led to more rapid postoperative recovery compared with that after the standard procedure. Third, because the new method requires fewer needle penetrations than the standard method, it most likely reduces the chances of infarction in extrahippocampal tissue. Finally, the new surgical approach may provide a mechanism for infusing agents into the hippocampus from a single cannula. Published (C) 2003 Wiley-Liss, Inc.dagger