Prions affect the appearance of other prions:: The story of [PIN+]
Prions affect the appearance of other prions:: The story of [PIN+]
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DOI：
10.1016/s0092-8674(01)00427-5
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发表时间：
2001-07-27
期刊：
影响因子：
64.5
通讯作者：
Liebman, SW
中科院分区：
文献类型：
--
作者：
Derkatch, IL;Bradley, ME;Liebman, SW
朊病毒是自我繁殖的蛋白质构象。最近的研究深入了解了朊病毒的传播，但它们最初是如何出现的尚不清楚。我们之前确定，酵母非孟德尔性状 [PIN+] 是 [PSI+] 朊病毒从头出现所必需的。在这里，我们证明由 Rnq1 或 Ure2 形成的朊病毒的存在足以使细胞形成 [PIN+]。因此，[PIN+] 可能是由不止一种朊病毒引起的。此外，对[PIN+]朊病毒的无偏功能筛选发现了已知的朊病毒基因URE2、拟议的朊病毒基因NEW1和九个新的候选朊病毒基因均携带朊病毒结构域。重要的是，Rnq1::GFP 朊病毒聚集体的从头出现也需要其他朊病毒的存在，这表明存在一种通用机制，通过异源朊病毒聚集体增强朊病毒的出现。
Prions are self-propagating protein conformations. Recent research brought insight into prion propagation, but how they first appear is unknown. We previously established that the yeast non-Mendelian trait [PIN+] is required for the de novo appearance of the [PSI+] prion. Here, we show that the presence of prions formed by Rnq1 or Ure2 is sufficient to make cells [PIN+]. Thus, [PIN+] can be caused by more than one prion. Furthermore, an unbiased functional screen for [PIN+] prions uncovered the known prion gene, URE2, the proposed prion gene, NEW1, and nine novel candidate prion genes all carrying prion domains. Importantly, the de novo appearance of Rnq1::GFP prion aggregates also requires the presence of other prions, suggesting the existence of a general mechanism by which the appearance of prions is enhanced by heterologous prion aggregates.