PEGylated synthetic surfactant vesicles (Niosomes): novel carriers for oligonucleotides
PEGylated synthetic surfactant vesicles (Niosomes): novel carriers for oligonucleotides
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DOI：
10.1007/s10856-007-3193-4
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发表时间：
2008-02-01
影响因子：
3.7
通讯作者：
Liang, Wenquan
中科院分区：
文献类型：
--
作者：
Huang, Yongzhuo;Chen, Jinliang;Liang, Wenquan
聚乙二醇（PEG）修饰的阳离子离子小体用于提高寡核苷酸（OND）的稳定性和细胞递送。由DC-Chol、PEG2000-DSPE和非离子表面活性剂span (R)组成的聚乙二醇化阳离子离子小体作为基因载体具有一定的优势。聚乙二醇化阳离子纳米体与OND的配合物呈中性zeta电位，粒径约为300 nm。peg修饰显著降低血清蛋白结合，阻止血清颗粒聚集。负载核酸药物对血清核酸酶的抗性增强。与阳离子乳质体相比，聚乙二醇化乳质体在血清中显示出更高的OND细胞摄取效率。因此，聚乙二醇化阳离子膜小体在储存和生理环境中具有稳定的理化性质，并且材料成本低、可获得性广，是提高OND体内药效的有前景的给药系统。
Polyethylene glycol (PEG) modified cationic niosomes were used to improve the stability and cellular delivery of oligonucleotides (OND). PEGylated cationic niosomes, composed of DC-Chol, PEG2000-DSPE and the non-ionic surfactant-Span (R), offer some advantages as gene carriers. Complexes of PEGylated cationic niosomes and OND showed a neutral zeta potential with particle size about 300 nm. PEG-modification significantly decreased the binding of serum protein and prevented particle aggregation in serum. The loaded nuclear acid drug exhibited increased resistance to serum nuclease. Compared with cationic niosomes, the PEGylated niosomes showed a higher efficiency of OND cellular uptake in serum. Therefore, in terms of their stable physiochemical properties in storage and physiological environment, as well as low-cost and widely available materials, PEGylated cationic niosomes are promising drug delivery systems for improved OND potency in vivo.