A Convenient and Versatile Amino-Acid-Boosted Biomimetic Strategy for the Nondestructive Encapsulation of Biomacromolecules within Metal-Organic Frameworks
A Convenient and Versatile Amino-Acid-Boosted Biomimetic Strategy for the Nondestructive Encapsulation of Biomacromolecules within Metal-Organic Frameworks
复制标题
一种方便且通用的氨基酸增强仿生策略，用于在金属有机框架内无损封装生物大分子
DOI：
10.1002/anie.201813060
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发表时间：
2019-01-28
影响因子：
16.6
通讯作者：
Ouyang, Gangfeng
中科院分区：
文献类型：
--
作者：
Chen, Guosheng;Huang, Siming;Ouyang, Gangfeng
在此，报道了一种氨基酸增强的仿生策略，该策略能够以超高的负载效率将多种蛋白质快速包裹或共包裹到微孔金属有机骨架(MOF)中。它依赖于通过类似金属硫蛋白的自组装在蛋白质周围加速形成核前簇。被包裹的蛋白质保持了它们的天然构象，而多孔MOF的结构限制赋予了酶优异的生物活性，即使在恶劣的条件下(例如，在蛋白水解剂或化学试剂存在或高温下)。此外，由于非破坏性和蛋白质表面电荷无关的优点，这种仿生策略在生物存储、酶级联和生物传感方面的可行性也得到了验证。人们认为，这种方便、通用的包埋技术在生物医学、催化、生物传感等重要领域有着广阔的应用前景。
Herein, an amino-acid-boosted biomimetic strategy is reported that enabled the rapid encapsulation, or co-encapsulation, of a broad range of proteins into microporous metal-organic frameworks (MOFs), with an ultrahigh loading efficiency. It relies on the accelerated formation of prenucleation clusters around proteins via a metallothionein-like self-assembly. The encapsulated proteins maintained their native conformations, and the structural confinement within porous MOFs endowed enzymes with excellent bioactivity, even in harsh conditions (e.g. in the presence of proteolytic or chemical agents or at high temperature). Furthermore, owing to the merits of nondestructive and protein surface charge-independent encapsulation, the feasibility of this biomimetic strategy for biostorage, enzyme cascades, and biosensing was also verified. It is believed that this convenient and versatile encapsulation strategy has great promise in the important fields of biomedicine, catalysis, and biosensing.