Chemical modification of viral ribonucleic acid. IX. The effect of ultraviolet irradiation on TMV-RNA and other polynucleotides.
Chemical modification of viral ribonucleic acid. IX. The effect of ultraviolet irradiation on TMV-RNA and other polynucleotides.
复制标题
病毒核糖核酸的化学修饰。
DOI：
10.1016/0042-6822(71)90117-6
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发表时间：
1971
期刊：
影响因子：
3.7
通讯作者：
B. Singer
中科院分区：
文献类型：
--
作者：
B. Singer
将UV照射多核苷酸后形成的尿苷水合物和嘧啶二聚体的量与照射后观察到的吸光度降低进行比较。在TMV-RNA的情况下，在高色和低色状态下进行照射，即，在水和10 - 3 MMg ~（2+）中，分析和吸光度数据与感染性损失进行了比较，从这些数据可以得出结论，TMV-RNA在水中吸光度的损失，以及poly U的损失，对应于UV产物的形成量。当在Mg 2+存在下进行与TMV-RNA的反应时，反应较慢，并且修饰的碱基的数量大于由吸光度损失计算的数量。当发生3-10个光化学事件时，发生一个失活事件。随着反应的进行，反应的效果变得不成比例地致命。二聚体与水合物的比例不影响致死率的发现表明，任一类型的光产物都可能代表灭活事件。
The amount of uridine hydrate and pyrimidine dimers formed upon UV irradiation of polynucleotides was compared with the absorbancy decrease observed upon irradiation. In the case of TMV-RNA, irradiation was performed in the hyperchromed and hypochromed state, i.e., in water and 10−3MMg2+, and the analytical and absorbancy data were compared with the losses of infectivity.From these data it was concluded that the loss of absorbancy of TMV-RNA in water, as well as that of poly U, corresponded to the amount of UV products formed. When the reaction with TMV-RNA was performed in presence of Mg2+, the reaction was slower and the number of modified bases greater than calculated from the absorbancy loss. One inactivating event occurred when 3–10 photochemical events had taken place. The effect of the reaction became proportionately less lethal as the reaction proceeded. The finding that the proportion of dimers to hydrates did not affect the lethality suggests that either type of photoproduct may represent an inactivating event.