CRISPR-mediated mutagenesis of the odorant receptor co-receptor (Orco) gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis
CRISPR-mediated mutagenesis of the odorant receptor co-receptor (Orco) gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis
复制标题
CRISPR介导的气味受体辅助受体（Orco）基因的突变破坏了橘小实蝇嗅觉介导的行为
DOI：
10.1111/1744-7917.12997
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发表时间：
2022
期刊：
影响因子：
4
通讯作者：
Jin-Jun Wang
中科院分区：
文献类型：
--
作者：
Li Xu;Hong-Bo Jiang;Kai-Yue Tang;Ying Yan;Marc F Schetelig;Jin-Jun Wang
嗅觉在昆虫的寄主定位、觅食、交配、产卵等行为中起着重要的作用。气味受体辅助受体（Orco）是一种强制性气味受体，在气味感知中不可或缺。本研究对东方实蝇（Bactrocera dorsalis（Hendel））的Orcogene基因进行了研究。嗅觉缺陷突变体通过使用CRISPR/Cas9系统编辑BdorOrcogene产生。触角电位（EAG）和嗅觉偏好测定证实，BdorOrco −/−突变果蝇对甲基丁香酚、β-香芹烯和乙酸乙酯的感知能力降低。产卵生物测定结果表明，苯并噻唑和1-辛烯-3-醇介导的BdorOrco −/−雌虫产卵量显著降低。此外，与野生型（WT）苍蝇相比，BdorOrco-/-突变苍蝇需要更长的时间来定位食物来源。综上所述，我们的数据表明Orco在B. dorsalis，它扩展了我们对昆虫Orco功能的理解。
Olfaction plays an essential role in insect behavior such as host location, foraging, mating, and oviposition. The odorant receptor co‐receptor (Orco) is an obligatory odorant receptor and indispensable in odor perception. Here, we characterized theOrcogene from the oriental fruit fly,Bactrocera dorsalis(Hendel), a notorious agriculture pest. The olfactory deficiency mutants were generated by editing theBdorOrcogene using the CRISPR/Cas9 system. Electroantennograms (EAG) and olfactory preference assays confirmed thatBdorOrco−/−mutant flies had reduced perception of methyl eugenol,β‐caryophyllene, and ethyl acetate. Oviposition bioassays showed that the eggs laid byBdorOrco−/−females mediated by benzothiazole and 1‐octen‐3‐ol were significantly decreased. In addition,BdorOrco−/−mutant flies took a significantly longer time to locate the food source compared with wild type (WT) flies. Altogether, our data indicated thatOrcois essential for multiple physiological processes inB. dorsalis, and it expands our understanding of the function of insectOrco.