A SWI/SNF- and INO80-dependent nucleosome movement at the INO1 promoter
A SWI/SNF- and INO80-dependent nucleosome movement at the INO1 promoter
复制标题
DOI：
10.1016/j.bbrc.2007.07.109
复制
发表时间：
2007-10-05
影响因子：
3.1
通讯作者：
Shen, Chang-Hui
中科院分区：
文献类型：
--
作者：
Ford, Jason;Odeyale, Oluwafemi;Shen, Chang-Hui
用间接末端标记技术研究了酵母INO1染色质的转录激活。在抑制条件下，INO1染色质被组织成有序的、重叠的核小体阵列。只有在SWI/SNF和INO80存在的诱导条件下，核小体位置才在启动子区域被破坏。缺少这两个重构体的突变体在抑制条件下表现出与野生型相同的定位模式。这表明这两个重构体对INO1启动子的局部核小体动员是负责的和必不可少的。核小体局部移动的区域与先前发现的组蛋白去乙酰化活性区域一致。根据这些发现，我们认为接受局部动员的核小体也是局部组蛋白修饰的靶点。(C)2007 Elsevier Inc.保留所有权利。
Transcriptional activation in yeast INO1 chromatin was studied using the indirect end-labeling technique. INO1 chromatin is organized into an ordered, overlapping nucleosomal array under repressing conditions. Nucleosome positions were only disrupted at the promoter region under inducing conditions in the presence of SWI/SNF and INO80. Mutants lacking either remodeler demonstrated identical positioning patterns as the wild type under repressing conditions. This indicates that these two remodelers are responsible and essential for local nucleosomal mobilization at the INO1 promoter. The area of local nucleosome movement is consistent with the previously identified region of histone deacetylation activity. In light of these findings, we suggest that nucleosomes subject to local mobilization are also targets for local histone modifications. (C) 2007 Elsevier Inc. All rights reserved.