Colocalization of serotonin and vesicular glutamate transporter 3-like immunoreactivity in the midbrain raphe of Syrian hamsters (Mesocricetus auratus)
Colocalization of serotonin and vesicular glutamate transporter 3-like immunoreactivity in the midbrain raphe of Syrian hamsters (Mesocricetus auratus)
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DOI：
10.1016/j.neulet.2005.10.033
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发表时间：
2006-02-13
影响因子：
2.5
通讯作者：
Scott, TJ
中科院分区：
文献类型：
--
作者：
Mintz, EM;Scott, TJ
囊泡谷氨酸转运蛋白3（VGLUT 3）的表达已被特异性定位于富含多巴胺能细胞的脑区。有人认为，这种转运蛋白可能有助于通过非突触机制调节细胞外谷氨酸浓度。在这项研究中，我们研究了共定位的囊泡谷氨酸转运蛋白3的免疫反应性与5-羟色胺的免疫反应性在背侧和正中中缝核的叙利亚仓鼠。将来自成年仓鼠的脑切片用荧光标记用于阿托宁-ir和VGLUT 3-ir，并使用共聚焦显微镜检查。结果表明，大多数中脑中缝的多巴胺能细胞也表达囊泡谷氨酸转运体3。此外，这些脑区的非多巴胺能细胞也显示出转运蛋白的免疫反应性。这些数据证实了以前的研究结果，囊泡谷氨酸转运蛋白3的表达，在大鼠的多巴胺能和非多巴胺能神经元。这些研究结果表明，囊泡谷氨酸转运蛋白3的位置可能是神经解剖位置的表达神经元的神经化学身份的功能。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Vesicular glutamate transporter 3 (VGLUT3) expression has been specifically localized to brain regions rich in serotonergic cells. It has been suggested that this transporter may contribute to the regulation of extracellular glutamate concentrations via a nonsynaptic mechanism. In this study, we examine the colocalization of vesicular glutamate transporter 3 immunoreactivity with serotonin immunoreactivity in the dorsal and median raphe nuclei of Syrian hamsters. Brain sections from adult hamsters were fluorescently labeled for serotonin-ir and VGLUT3-ir and examined using confocal microscopy. The results indicate that most serotonergic cells of the midbrain raphe also expressed vesicular glutamate transporter 3. In addition, nonserotonergic cells in these brain regions also show immunoreactivity for the transporter. These data confirm previous findings of vesicular glutamate transporter 3 expression in serotonergic and nonserotonergic neurons in rats. These findings suggest that the location of vesicular glutamate transporter 3 may be as much a function of neuroanatomical location as of the neurochemical identity of the expressing neurons. (c) 2005 Elsevier Ireland Ltd. All rights reserved.