Effect of colcemid exposure and methanol acetic acid fixation on human metaphase chromosome structure.
Effect of colcemid exposure and methanol acetic acid fixation on human metaphase chromosome structure.
复制标题
秋水酰胺暴露和甲醇乙酸固定对人中期染色体结构的影响。
DOI：
10.1111/j.1601-5223.1979.tb01306.x
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发表时间：
2009
期刊：
影响因子：
2.7
通讯作者：
M. Erlandsen
中科院分区：
文献类型：
--
作者：
M. Rønne;O. Andersen;M. Erlandsen
当培养细胞且未暴露秋水仙胺时，检查人淋巴样细胞在有丝分裂亚期、前期、前期、中期和后期的分布。染色体编号平均长度较高。我发现过夜固定后比用 3:1 甲醇乙酸固定剂短时间固定后要好。过夜固定后，在未进行任何固定后带诱导的载玻片上观察到带状染色体的概率高于短时间固定后的概率。由于中期细胞的甲醇乙酸固定导致组蛋白提取的增加导致平均染色体长度增加以及染色体显带更频繁，因此表明不仅非组蛋白而且组蛋白也参与染色体带的形成。
在未接触秋水酰胺的情况下收获的细胞中。大多数观察到的染色体是带状中期染色体。这表明，只要在生长培养基中添加RNA或蛋白质合成抑制剂，染色体浓缩过程就会被延迟或部分抑制，只要暴露于秋水仙胺的细胞仍停留在中期边界，游离的-SH基团可用于氧化，染色体浓缩过程就会继续进行。
Distribution of human lymphoid cells in the mitotic subphases, prophase, prometaphase, metaphase and anatelophase, was examined, when cells had been cultured and without colcemid exposure. A higher average length of chromosome no. I was found after overnight fixation than after short time fixation with 3:1 methanol acetic acid fixative. The probability of observing banded chromosomes on slides that had not been exposed to any postfixational band induction was higher after overnight fixation than after short time fixation. Since an increase in the extraction of histones caused by the methanol acetic acid fixation of metaphase cells led to an increase of average chromosome length as well as more frequent banding of chromosomes, it is suggested that not only non-histone proteins but also histones are involved in the formation of chromosome bands.
In cells harvested without having been exposed to colcemid. most of the observed chromosomes were banded prometaphase chromosomes. It is suggested that the chromosome condensation process, which is delayed or partly inhibited when inhibitors of RNA or protein synthesis are added to the growth medium, continues as long as colcemid exposed cells remain arrested at the meta-anaphase border with free -SH groups available for oxidation.