The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
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DOI：
10.1101/2023.01.05.522848
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发表时间：
2023-01
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影响因子：
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通讯作者：
J. Almeida;L. Perez-Fons;M. Drapal;Kit Xi Liew;P. Fraser
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文献类型：
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作者：
J. Almeida;L. Perez-Fons;M. Drapal;Kit Xi Liew;P. Fraser
质体球 (PG) 是植物脂蛋白区室，存在于质体细胞器中。它们参与亲脂性代谢物的形成和/或储存。纤维蛋白 (FBN) 是主要的 PG 相关蛋白之一，在成熟水果和花朵中富含类胡萝卜素的有色体中含量特别丰富。为了解决不同 FBN 对类异戊二烯隔离和 PG 功能的贡献，采用了多重基因编辑方法。对番茄中主要 PG 相关 FBN（即 SlFBN1、SlFBN2a、SlFBN4、SlFBN7a）的单一和高阶 fbn 突变体的分析揭示了功能冗余。高阶 fbn 突变体表现出与异常类异戊二烯积累以及异常 PG 形成和形态相关的表型。脂质组学分析强调了脂质代谢的更广泛变化。还观察到旁系同源特异性作用，包括对特定类异戊二烯（例如质体色醇）的调节和 SlFBN7a 对质体酯化能力的控制。总的来说，结果支持 SlFBN 在 PG 中的结构和调节作用。我们的研究结果揭示了植物细胞代谢区室化的基本方面以及脂蛋白颗粒对其质体代谢/生理学的重要性。意义说明 在成熟番茄果实和花朵的有色体中，质体球 (PG) 由于其在代谢、发育转变和环境适应中的功能参与，与几个重要的生物技术特征相关。 FIBRILLINS (FBN) 是一个多基因蛋白质家族，它们都是 PG 的主要组成部分。使用多重 CRISPR-Cas9 方法，已开发出单一和高阶 fbn 突变体。 FBN 多基因家族成员之间的功能冗余是明显的，而且旁系同源的特定功能/影响也很明显。 fbn 突变体中质体球形成异常和脂质代谢改变很明显。该资源的表征揭示了 FBN 的功能作用及其在 PG 形成中的作用。这一战略为开发营养增强型和气候适应型作物提供了新的潜力。
Plastoglobuli (PG) are plant lipoprotein compartments, present in plastid organelles. They are involved in the formation and/or storage of lipophilic metabolites. FIBRILLINs (FBN) are one of the main PG-associated proteins and are particularly abundant in carotenoid-enriched chromoplasts found in ripe fruits and flowers. To address the contribution of different FBNs to isoprenoid sequestration and PG function, a multiplex gene editing approach was undertaken. Analysis of single and high-order fbn mutants for the major PG-related FBNs in tomato, namely SlFBN1, SlFBN2a, SlFBN4, SlFBN7a, revealed functional redundancy. High order fbn mutants displayed phenotypes associated with abnormal isoprenoid accumulation, and aberrant PG formation and morphology. Lipidomic analysis highlighted broader changes in lipid metabolism. Paralog-specific roles were also observed and included the regulation of specific isoprenoids (e.g., plastochromanol) and control of plastidial esterification capability by SlFBN7a. Collectively, the results support both structural and regulatory roles of SlFBNs in PGs. Our findings expose fundamental aspects of metabolic compartmentalisation in plant cells and the importance of lipoprotein particles for their plastid metabolism/physiology. Significance statement In the chromoplast of ripe tomato fruit and flower, plastoglobuli (PGs) are associated with several important biotechnological traits, due to their functional involvement in metabolism, developmental transitions, and environmental adaption. FIBRILLINS (FBN) are a multigene family of proteins that are collectively major components of the PG. Using a multiplex CRISPR-Cas9 approach single and high-order fbn mutants have been developed. Functional redundancy amongst the members of the FBN multigene family was evident, but also paralog specific functions/influence. Aberrant plastoglobuli formation and altered lipid metabolism are evident among fbn mutants. Characterisation of this resource has shed light on the functional role of FBN and their role in PG formation. This strategy offers new potential for the development of nutritional enhanced and climate resilient crops.