A Mouse Model for Laser-induced Choroidal Neovascularization
A Mouse Model for Laser-induced Choroidal Neovascularization
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DOI：
10.3791/53502
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发表时间：
2015-12-01
影响因子：
1.2
通讯作者：
Fawzi, Amani A.
中科院分区：
文献类型：
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作者：
Shah, Ronil S.;Soetikno, Brian T.;Fawzi, Amani A.
激光诱导的小鼠脉络膜新生血管（CNV）模型是研究新生血管性年龄相关性黄斑变性（AMD）的重要模型。通过对RPE和Bruch膜进行靶向激光损伤，该过程诱导血管生成，模拟在新生血管性AMD中观察到的标志性病理学。首先在非人类灵长类动物中开发，激光诱导的CNV模型已经在许多其他物种中实施，其中最近的是小鼠。小鼠实验有利地更具成本效益，实验可以在快得多的时间轴上执行，并且它们允许使用各种转基因模型。然而，小鼠眼睛的微型尺寸在执行该程序时构成了特别的挑战。操纵眼睛来使视网膜可视化需要练习精细的灵巧技能以及同时的手-眼-脚协调来操作激光。然而，一旦掌握了该模型，可以应用于研究新生血管性AMD的许多方面，如分子机制，遗传操作的影响，和药物治疗效果。野生型小鼠眼睛是健康的，并且脉络膜视网膜环境不模仿人类AMD的病理变化。此外，损伤诱导的血管生成并不反映与年龄相关的慢性疾病状态中发生的血管生成相同的途径，如在AMD. Despite其缺点，激光诱导的CNV模型是目前可用于研究新生血管性AMD的衰弱病理学的最佳方法之一。它的实施使人们对AMD的发病机制有了更深入的了解，并有助于开发目前可用的许多AMD疗法。
The mouse laser-induced choroidal neovascularization (CNV) model has been a crucial mainstay model for neovascular age-related macular degeneration (AMD) research. By administering targeted laser injury to the RPE and Bruch's membrane, the procedure induces angiogenesis, modeling the hallmark pathology observed in neovascular AMD.First developed in non-human primates, the laser-induced CNV model has come to be implemented into many other species, the most recent of which being the mouse. Mouse experiments are advantageously more cost-effective, experiments can be executed on a much faster timeline, and they allow the use of various transgenic models. The miniature size of the mouse eye, however, poses a particular challenge when performing the procedure. Manipulation of the eye to visualize the retina requires practice of fine dexterity skills as well as simultaneous hand-eye-foot coordination to operate the laser. However, once mastered, the model can be applied to study many aspects of neovascular AMD such as molecular mechanisms, the effect of genetic manipulations, and drug treatment effects.The laser-induced CNV model, though useful, is not a perfect model of the disease. The wild-type mouse eye is otherwise healthy, and the chorio-retinal environment does not mimic the pathologic changes in human AMD. Furthermore, injury-induced angiogenesis does not reflect the same pathways as angiogenesis occurring in an age-related and chronic disease state as in AMD.Despite its shortcomings, the laser-induced CNV model is one of the best methods currently available to study the debilitating pathology of neovascular AMD. Its implementation has led to a deeper understanding of the pathogenesis of AMD, as well as contributing to the development of many of the AMD therapies currently available.