Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts
Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts
复制标题
DOI：
10.1016/j.jnutbio.2009.04.005
复制
发表时间：
2010-07-01
影响因子：
5.6
通讯作者：
Matsuo, Takashi
中科院分区：
文献类型：
--
作者：
Hosokawa, Yoshitaka;Hosokawa, Ikuko;Matsuo, Takashi
CXC趋化因子配体10（CXCL 10）在Th 1细胞的募集中起关键作用，因此在牙周病的发展中起关键作用。表没食子儿茶素没食子酸酯（EGCG）和表儿茶素没食子酸酯（ECG）是源自绿色茶的主要儿茶素，具有多种有益作用，但儿茶素对人牙龈成纤维细胞（HGF）产生CXCL 10的影响尚不清楚。在这项研究中，我们研究了EGCG和ECG抑制制瘤素M（OSM）诱导的HGF产生CXCL 10的机制。HGF组成型表达糖蛋白130和OSM受体β（OSMR β），它们是OSM受体。OSM以浓度依赖性方式增加CXCL 10的产生。EGCG和ECG阻止OSM介导的HGF产生CXCL 10。p38丝裂原活化蛋白激酶、c-Jun N-末端激酶（INK）、磷脂酰肌醇-3-OH激酶和信号转导和转录激活因子（STAT）3的抑制剂可降低OSM诱导的CXCL 10产生。EGCG显著抑制OSM诱导的JNK、Akt（Ser 473）和STAT 3（Tyr 705和Ser 727）的磷酸化。ECG可抑制JNK和Akt（Ser 473）的磷酸化。此外，EGCG和ECG减弱了HGF上的OSMR β表达。这些数据提供了一种新的机制，通过这种机制，绿色茶类黄酮，儿茶素，可以在牙周病中提供直接的好处。(C)2010年爱思唯尔公司All rights reserved.
CXC chemokine ligand 10 (CXCL10) plays a pivotal role in the recruitment of Th1 cells and, thus, in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins derived from green tea, have multiple beneficial effects, but the effects of catechins on CXCL10 production from human gingival fibroblasts (HGFs) is not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit oncostatin M (OSM)-induced CXCL10 production in HGFs. HGFs constitutively expressed glycoprotein 130 and OSM receptor beta (OSMR beta), which are OSM receptors. OSM increased CXCL10 production in a concentration-dependent manner. EGCG and ECG prevented OSM-mediated CXCL10 production by HGFs. Inhibitors of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase (INK), phosphatidylinositol-3-OH kinase and signal transducer and activator of transcription (STAT)3 decreased OSM-induced CXCL10 production. EGCG significantly prevented OSM-induced phosphorylation of JNK, Akt (Ser473) and STAT3 (Tyr705 and Ser727). ECG prevented phosphorylation of INK and Akt (Ser473). In addition, EGCG and ECG attenuated OSMR beta expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids, catechins, can provide direct benefits in periodontal disease. (C) 2010 Elsevier Inc. All rights reserved.