Apoptotic body engulfment by hepatic stellate cells promotes their survival by the JAK/STAT and Akt/NF-kappaB-dependent pathways.
Apoptotic body engulfment by hepatic stellate cells promotes their survival by the JAK/STAT and Akt/NF-kappaB-dependent pathways.
复制标题
肝星状细胞吞噬的凋亡人体通过JAK/STAT和AKT/NF-KAPPAB依赖性途径促进其生存。
DOI：
10.1016/j.jhep.2009.03.024
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发表时间：
2009-07
影响因子：
25.7
通讯作者：
Török NJ
中科院分区：
文献类型：
--
作者：
Jiang JX;Mikami K;Venugopal S;Li Y;Török NJ
We have previously shown that phagocytosis of apoptotic bodies (AB) by hepatic stellate cells (HSC) is profibrogenic. As HSC survival is central to the progression of liver fibrosis, our goal was to investigate if phagocytosis induces HSC survival. Apoptosis of phagocytosing HSC was studied in the presence of known apoptotic agents. The JAK/STAT and PI3K/Akt dependent pathways, NF-κB activation and expression of the anti-apoptotic proteins Mcl-1 and A1 were evaluated. Apoptosis was assessed after blocking A1 by an siRNA approach. Phagocytosing HSC were resistant to FasL/cycloheximide or TRAIL-induced apoptosis. Inhibition of the JAK/STAT or PI3K-mediated pathways induced apoptosis of HSC. Phagocytosis induced JAK1/STAT3 phosphorylation, and this was prevented by inhibiting JAK. Translocation of STAT3 to the nucleus was also blocked by JAK inhibition. Mcl-1 expression was upregulated in a JAK-dependent manner. PI3K-dependent phosphorylation of Akt depended on NADPH oxidase activity and superoxide production. NF-κB activation and subsequent upregulation of A1 was observed, and A1 inhibition induced apoptosis of HSC. Phagocytosis of AB promotes HSC survival by two pathways, of which the A1 dependent is more significant. This represents a new mechanism by which engulfment of AB contributes to the propagation of liver fibrosis.
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影响因子：
15.9
作者：
Epling-Burnette, PK;Liu, JH;Loughran, TP
通讯作者：
Loughran, TP
影响因子：
8
作者：
Gong, LJ;Li, YW;Sarkar, FH
通讯作者：
Sarkar, FH
影响因子：
13.5
作者：
Gong, WR;Pecci, A;Gressner, AM
通讯作者：
Gressner, AM
DOI：
10.1042/bss2007c06
发表时间：
2007-01-01
期刊：
CELL BIOLOGY OF INOSITOL LIPIDS AND PHOSPHATES
影响因子：
--
作者：
Hawkins, Phillip T.;Davidson, Keith;Stephens, Len R.
通讯作者：
Stephens, Len R.
影响因子：
29.4
作者：
Isomoto, Hajime;Mott, Justin L.;Gores, Gregory J.
通讯作者：
Gores, Gregory J.