Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways
Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways
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DOI：
10.1073/pnas.1110416108
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发表时间：
2011-12-13
影响因子：
11.1
通讯作者：
Brunet, Jean-Francois
中科院分区：
文献类型：
--
作者：
D'Autreaux, Fabien;Coppola, Eva;Brunet, Jean-Francois
消化、呼吸和心血管器官反馈调节所需的味觉和大多数感觉输入由位于三个脑神经节(膝状神经节、岩神经节和结状神经节)的所谓“内脏”感觉神经元传递到中枢神经系统，并通过位于孤束核的中继性感觉神经元整合到后脑。内脏感觉神经节和孤束核的形成都依赖于泛内脏同源结构域转录因子PHOX2B，该因子也是内脏传出神经元所必需的。我们在这里通过基因追踪PHOX2B(+)细胞表明，在缺乏蛋白质的情况下，许多内脏感觉神经元(一级和二级)存活。然而，它们的命运--包括分子特征、细胞位置和轴突投射--类似于位于三叉神经节、上神经节、颈静脉神经节和三叉神经感觉核的躯体感觉神经元(一级和二级)，从口面部传递触觉和痛觉。因此，躯体和内脏的颅脑感觉通路是相关的，PHOX2B是从前者到后者的发育转换。
Taste and most sensory inputs required for the feedback regulation of digestive, respiratory, and cardiovascular organs are conveyed to the central nervous system by so-called "visceral" sensory neurons located in three cranial ganglia (geniculate, petrosal, and nodose) and integrated in the hindbrain by relay sensory neurons located in the nucleus of the solitary tract. Visceral sensory ganglia and the nucleus of the solitary tract all depend for their formation on the pan-visceral homeodomain transcription factor Phox2b, also required in efferent neurons to the viscera. We show here, by genetically tracing Phox2b(+) cells, that in the absence of the protein, many visceral sensory neurons (first-and second-order) survive. However, they adopt a fate-including molecular signature, cell positions, and axonal projections-akin to that of somatic sensory neurons (first-and second-order), located in the trigeminal, superior, and jugular ganglia and the trigeminal sensory nuclei, that convey touch and pain sensation from the orofacial region. Thus, the cranial sensory pathways, somatic and visceral, are related, and Phox2b serves as a developmental switch from the former to the latter.